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ICAM1 抗体

The 兔 多克隆 anti-ICAM1 antibody (ABIN465660) specifically detects ICAM1 in WB 和 ELISA. The antibody is reactive with 人 samples.
产品编号 ABIN465660
发货至: 中国
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北京 101111
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Room 801-803
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Quick Overview for ICAM1 抗体 (ABIN465660)

抗原

See all ICAM1 抗体
ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

适用

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多克隆

标记

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This ICAM1 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA
  • 特异性

    Human ICAM-1

    纯化方法

    Immunoaffinity purified

    免疫原

    CHO cell derived recombinant Human ICAM-1.

    Type of Immunogen: Cell extract
  • 应用备注

    Approved: ELISA (0.5 - 2 μg/mL), WB (0.1 - 0.2 μg/mL)

    Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal adenocarcinoma (moderately differentiated). The recommended concentration is 0.25 mg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human ICAM-1 (10 ng/mL), a concentration of 8 u/mL of this antibody is required. ELISA: To detect Human ICAM-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human ICAM-1 (LS-C104787) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human ICAM-1. Western Blot: To detect Human ICAM-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human ICAM-1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Sterile water

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS,  pH 7.2

    注意事项

    Avoid freeze/thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原

    ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

    别名

    ICAM1 / CD54

    背景

    Name/Gene ID: ICAM1
    Family: ICAM

    Synonyms: ICAM1, BB2, CD54 antigen, CD54, ICAM-1, p3.58

    基因ID

    3383

    UniProt

    P05362

    途径

    Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling
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