PDGF-BB Homodimer 抗体
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- 抗原
- PDGF-BB Homodimer
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), ELISA, Neutralization (Neut)
- 特异性
- Human PDGF-BB
- 纯化方法
- Immunoaffinity purified
- 免疫原
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E.coli derived recombinant Human PDGF-BB.
Type of Immunogen: Cell extract
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anti-PDGF-BB Homodimer (AA 151-241) antibody
适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-PDGF-BB Homodimer (AA 82-190) antibody适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-PDGF-BB Homodimer (AA 21-241) antibody适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-PDGF-BB Homodimer (N-Term) antibody适用: 人, 小鼠, 大鼠, Cow, 绵羊, Bat, 犬, 豚鼠, 兔, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-PDGF-BB Homodimer (AA 82-190) antibody适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal 4# unconjugated
anti-PDGF-BB Homodimer (AA 5-225) antibody适用: 大鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Approved: ELISA, Neut (3 - 4 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1 μg/mL overnight at 4 °C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hPDGF-BB (2.5 ng/mL), a concentration of 0.03-0.04 μg/mL of this antibody is required. ELISA: To detect hPDGF-BB by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PDGF-BB (LS-C104638) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPDGF-BB. Western Blot: To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Sterile water to a concentration of 0.1-1.0 mg/mL
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, pH 7.2
- 注意事项
- Avoid freeze/thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- 抗原
- PDGF-BB Homodimer
- 别名
- PDGF-BB
- 别名
- PDGF-2 antibody, PDGF2 antibody, SIS antibody, SSV antibody, c-sis antibody, PDGF-B antibody, Sis antibody, platelet derived growth factor subunit B antibody, platelet derived growth factor, B polypeptide antibody, PDGFB antibody, Pdgfb antibody
- 背景
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Name/Gene ID: PDGFB
Family: PDGF
Synonyms: PDGFB, Becaplermin, PDGF-BB, PDGF-b, SSV, PDGF subunit B, PDGF-2, PDGF2, Proto-oncogene c-Sis, SIS, C-sis, PDGF, B chain - 基因ID
- 5155
- UniProt
- P01127
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