KIT Ligand 抗体
Quick Overview for KIT Ligand 抗体 (ABIN465573)
抗原
See all KIT Ligand (KITLG) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Human SCF
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纯化方法
- Immunoaffinity purified
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免疫原
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E.coli derived recombinant Human SCF.
Type of Immunogen: Cell extract
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应用备注
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Approved: ELISA (0.5 - 2 μg/mL), Neut (8 - 12 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 0.5 μg/mL-1 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Optimal results for these conditions were achieved with enzyme induced antigen retrieval (proteinase K) at room temperature. Optimal concentrations and conditions may vary. *Additional Immunostaining data available. Please contact Tech Support for information. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hSCF (10.00 ng/mL), a concentration of 0.64-0.96 μg/mL of this antibody is required. ELISA: To detect hSCF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human SCF (LS-C104672) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hSCF. Western Blot: To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCF is 1.5-3 ng/lane, under either reducing or non-reducing conditions. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile water
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, pH 7.2
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注意事项
- Avoid freeze/thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- KIT Ligand (KITLG)
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别名
- KITLG / SCF
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背景
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Name/Gene ID: KITLG
Synonyms: KITLG, C-Kit ligand, KIT ligand, Kitl, KL-1, SHEP7, SF, Steel factor, Stem cell factor, SCF, FPH2, Mast cell growth factor, MGF -
基因ID
- 4254
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UniProt
- P21583
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
抗原
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