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KIT Ligand 抗体

This anti-KIT Ligand antibody is a 兔 多克隆 antibody detecting KIT Ligand in WB, ELISA 和 Neut. Suitable for 人.
产品编号 ABIN465573
发货至: 中国

Quick Overview for KIT Ligand 抗体 (ABIN465573)

抗原

See all KIT Ligand (KITLG) 抗体
KIT Ligand (KITLG)

适用

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宿主

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克隆类型

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多克隆

标记

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This KIT Ligand antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Neutralization (Neut)
  • 特异性

    Human SCF

    纯化方法

    Immunoaffinity purified

    免疫原

    E.coli derived recombinant Human SCF.

    Type of Immunogen: Cell extract
  • 应用备注

    Approved: ELISA (0.5 - 2 μg/mL), Neut (8 - 12 μg/mL), WB

    Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 0.5 μg/mL-1 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Optimal results for these conditions were achieved with enzyme induced antigen retrieval (proteinase K) at room temperature. Optimal concentrations and conditions may vary. *Additional Immunostaining data available. Please contact Tech Support for information. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hSCF (10.00 ng/mL), a concentration of 0.64-0.96 μg/mL of this antibody is required. ELISA: To detect hSCF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human SCF (LS-C104672) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hSCF. Western Blot: To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Sterile water

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS,  pH 7.2

    注意事项

    Avoid freeze/thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原

    KIT Ligand (KITLG)

    别名

    KITLG / SCF

    背景

    Name/Gene ID: KITLG

    Synonyms: KITLG, C-Kit ligand, KIT ligand, Kitl, KL-1, SHEP7, SF, Steel factor, Stem cell factor, SCF, FPH2, Mast cell growth factor, MGF

    基因ID

    4254

    UniProt

    P21583

    途径

    RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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