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PDGF-BB Homodimer 抗体 (Biotin)

The 兔 多克隆 anti-PDGF-BB Homodimer antibody is suitable to detect PDGF-BB Homodimer in samples from 人. It has been validated for WB 和 ELISA.
产品编号 ABIN465516
发货至: 中国
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Beijing Economic Technological Development Area
Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for PDGF-BB Homodimer 抗体 (Biotin) (ABIN465516)

抗原

PDGF-BB Homodimer

适用

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宿主

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克隆类型

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多克隆

标记

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Biotin

应用范围

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Western Blotting (WB), ELISA
  • 特异性

    Human PDGF-BB

    纯化方法

    Immunoaffinity purified

    免疫原

    E.coli derived recombinant Human PDGF-BB.

    Type of Immunogen: Cell extract
  • 应用备注

    Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

    Usage: ELISA: To detect hPDGF-BB by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hPDGF-BB. To detect hPDGF-BB by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human PDGF-BB (LS-C104675) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPDGF-BB. Western Blot: To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Sterile PBS containing 0.1 % BSA

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS,  pH 7.2

    注意事项

    Avoid freeze/thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原

    PDGF-BB Homodimer

    别名

    PDGF-BB

    背景

    Name/Gene ID: PDGFB
    Family: PDGF

    Synonyms: PDGFB, Becaplermin, PDGF-BB, PDGF-b, SSV, PDGF subunit B, PDGF-2, PDGF2, Proto-oncogene c-Sis, SIS, C-sis, PDGF, B chain

    基因ID

    5155

    UniProt

    P01127
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