WISP2 抗体
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北京 101111
Quick Overview for WISP2 抗体 (ABIN465385)
抗原
See all WISP2 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Human CTGFL/WISP-2
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纯化方法
- Immunoaffinity purified
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免疫原
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E.coli derived recombinant Human CTGFL/WISP-2.
Type of Immunogen: Cell extract
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应用备注
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Approved: ELISA, WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.5 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Additional protein blocks were used in conjunction with the secondary reagents. Optimal concentrations and conditions may vary. ELISA: To detect hCTGFL/WISP-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CTGFL/WISP-2 (LS-C104595) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hCTGFL/WISP-2. Western Blot: To detect hCTGFL/WISP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGFL/WISP-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile water
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, pH 7.2
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注意事项
- Avoid freeze/thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- WISP2 (WNT1 Inducible Signaling Pathway Protein 2 (WISP2))
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别名
- WISP2
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背景
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Name/Gene ID: WISP2
Synonyms: WISP2, CCN5, CCN family member 5, CTGFL, CT58, CTGF-L, WISP-2 -
基因ID
- 8839
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UniProt
- O76076
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途径
- WNT signaling, Growth Factor Binding
抗原
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