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WISP2 抗体

This 兔 多克隆 antibody specifically detects WISP2 in WB 和 ELISA. It exhibits reactivity toward 人.
产品编号 ABIN465385
发货至: 中国
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Quick Overview for WISP2 抗体 (ABIN465385)

抗原

See all WISP2 抗体
WISP2 (WNT1 Inducible Signaling Pathway Protein 2 (WISP2))

适用

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  • 5
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宿主

  • 38
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克隆类型

  • 39
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多克隆

标记

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This WISP2 antibody is un-conjugated

应用范围

  • 33
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Western Blotting (WB), ELISA
  • 特异性

    Human CTGFL/WISP-2

    纯化方法

    Immunoaffinity purified

    免疫原

    E.coli derived recombinant Human CTGFL/WISP-2.

    Type of Immunogen: Cell extract
  • 应用备注

    Approved: ELISA, WB (0.1 - 0.2 μg/mL)

    Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.5 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Additional protein blocks were used in conjunction with the secondary reagents. Optimal concentrations and conditions may vary. ELISA: To detect hCTGFL/WISP-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CTGFL/WISP-2 (LS-C104595) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hCTGFL/WISP-2. Western Blot: To detect hCTGFL/WISP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGFL/WISP-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Sterile water

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS,  pH 7.2

    注意事项

    Avoid freeze/thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原

    WISP2 (WNT1 Inducible Signaling Pathway Protein 2 (WISP2))

    别名

    WISP2

    背景

    Name/Gene ID: WISP2

    Synonyms: WISP2, CCN5, CCN family member 5, CTGFL, CT58, CTGF-L, WISP-2

    基因ID

    8839

    UniProt

    O76076

    途径

    WNT signaling, Growth Factor Binding
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