Midkine 抗体 (Biotin)
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北京 101111
Quick Overview for Midkine 抗体 (Biotin) (ABIN465383)
抗原
See all Midkine (MDK) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Human Midkine
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纯化方法
- Immunoaffinity purified
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免疫原
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E.coli derived recombinant Human Midkine.
Type of Immunogen: Cell extract
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应用备注
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Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: ELISA: To detect hMidkine by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hMidkine. To detect hMidkine by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human Midkine (LS-B8221) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMidkine. Western Blot: To detect hMidkine by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMidkine is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile PBS containing 0.1 % BSA
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, pH 7.2
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注意事项
- Avoid freeze/thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))
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别名
- MDK / Midkine
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背景
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Name/Gene ID: MDK
Synonyms: MDK, ARAP, MK, MK1, NEGF2, Midkine, Retinoic acid inducible factor -
基因ID
- 4192
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UniProt
- P21741
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途径
- RTK signaling, M Phase, Skeletal Muscle Fiber Development
抗原
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