IL-10 抗体
Quick Overview for IL-10 抗体 (ABIN465277)
抗原
See all IL-10 (IL10) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Mouse IL-10
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纯化方法
- Protein G purified
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免疫原
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E.coli derived recombinant Murine IL-10.
Type of Immunogen: Cell extract -
亚型
- IgG2a
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应用备注
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Approved: ELISA (4 - 8 μg/mL), Neut (3 - 5 μg/mL), WB (1 - 2 μg/mL)
Usage: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Murine IL-10 (0.5 ng/mL), a concentration of 3-5 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4-8 μg/mL of this antibody will detect at least 1600 pg/mL of recombinant murine IL-10 when used with biotinylated antigen affinity purified anti-murine IL-10 (LS-C104822) as the detection antibody at a concentration of approximately 0.5-1 μg/mL. Western Blot: To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1-2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2-4 ng/lane, under reducing or non-reducing conditions. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile water
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS
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注意事项
- Avoid freeze/thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- IL-10 (IL10) (Interleukin 10 (IL10))
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别名
- IL10
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背景
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Name/Gene ID: IL10
Family: Hormone
Synonyms: IL10, CSIF, GVHDS, IL-10, IL10A, Interleukin-10, Interleukin 10, TGIF -
基因ID
- 3586
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UniProt
- P22301
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
抗原
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