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IL-10 抗体

This 大鼠 单克隆 antibody specifically detects IL-10 in WB, ELISA 和 Neut. It exhibits reactivity toward 小鼠.
产品编号 ABIN465277
发货至: 中国
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Quick Overview for IL-10 抗体 (ABIN465277)

抗原

See all IL-10 (IL10) 抗体
IL-10 (IL10) (Interleukin 10 (IL10))

适用

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小鼠

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大鼠

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单克隆

标记

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This IL-10 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Neutralization (Neut)

克隆位点

15B9-1-2
  • 特异性

    Mouse IL-10

    纯化方法

    Protein G purified

    免疫原

    E.coli derived recombinant Murine IL-10.

    Type of Immunogen: Cell extract

    亚型

    IgG2a
  • 应用备注

    Approved: ELISA (4 - 8 μg/mL), Neut (3 - 5 μg/mL), WB (1 - 2 μg/mL)

    Usage: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Murine IL-10 (0.5 ng/mL), a concentration of 3-5 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4-8 μg/mL of this antibody will detect at least 1600 pg/mL of recombinant murine IL-10 when used with biotinylated antigen affinity purified anti-murine IL-10 (LS-C104822) as the detection antibody at a concentration of approximately 0.5-1 μg/mL. Western Blot: To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1-2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2-4 ng/lane, under reducing or non-reducing conditions.

    说明

    Target Species of Antibody: Mouse

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Sterile water

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS

    注意事项

    Avoid freeze/thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原

    IL-10 (IL10) (Interleukin 10 (IL10))

    别名

    IL10

    背景

    Name/Gene ID: IL10
    Family: Hormone

    Synonyms: IL10, CSIF, GVHDS, IL-10, IL10A, Interleukin-10, Interleukin 10, TGIF

    基因ID

    3586

    UniProt

    P22301

    途径

    Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
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