Nerve Growth Factor 抗体
Quick Overview for Nerve Growth Factor 抗体 (ABIN465164)
抗原
See all Nerve Growth Factor (NGF) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Human beta-NGF
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纯化方法
- Protein G purified
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免疫原
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E.coli derived recombinant Human beta-NGF.
Type of Immunogen: Cell extract -
亚型
- IgG2b
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应用备注
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Approved: ELISA (2 - 4 μg/mL), Neut (2.7 - 4 μg/mL), WB (4 - 8 μg/mL)
Usage: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human beta-NGF (10 ng/mL), a concentration of 2.7-4 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of this antibody will detect at least 200 pg/mL of recombinant Human beta-NGF when used with biotinylated antigen affinity purified anti-Human beta-NGF (LS-C104735) as the detection antibody at a concentration of approximately 0.5-1 μg/mL. Western Blot: To detect Human beta-NGF by Western Blot analysis this antibody can be used at a concentration of 4-8 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human beta-NGF is 4-8 ng/lane, under reducing or non-reducing conditions. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile water
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS
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注意事项
- Avoid freeze/thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Nerve Growth Factor (NGF)
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别名
- NGF
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背景
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Name/Gene ID: NGF
Family: NGF Beta
Synonyms: NGF, Beta-nerve growth factor, ProNGF, Beta-NGF, HSAN5, NGFB -
基因ID
- 4803
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UniProt
- P01138
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途径
- Regulation of Cell Size
抗原
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