BMP4 抗体
Quick Overview for BMP4 抗体 (ABIN465142)
抗原
See all BMP4 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Human BMP-4
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纯化方法
- Protein G purified
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免疫原
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E.coli derived recombinant mature Human BMP-4.
Type of Immunogen: Cell extract -
亚型
- IgG2b
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应用备注
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Approved: ELISA (4 - 8 μg/mL), Neut (0.4 - 0.6 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained HepG2 cells. The primary antibody was incubated at 3 μg/mL overnight at 4 °C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information courtesy of the Cell Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human BMP-4 (5 ng/mL), a concentration of 0.4-0.6 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4-8 μg/mL of this antibody will detect at least 2000 pg/mL of recombinant human BMP-4 when used in conjunction with compatible secondary reagents. Western Blot: To detect hBMP-4 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-4 is 2-4 ng/lane, under non-reducing conditions. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile water
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS
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注意事项
- Avoid freeze/thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- BMP4 (Bone Morphogenetic Protein 4 (BMP4))
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别名
- BMP4 / BMP-4
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背景
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Name/Gene ID: BMP4
Family: TGF beta
Synonyms: BMP4, BMP-2B, BMP-4, BMP2B, BMP2B1, Bone morphogenetic protein 2B, Bone morphogenetic protein 4, DVR4, MCOPS6, OFC11, Zyme -
基因ID
- 652
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UniProt
- P12644
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途径
- Steroid Hormone Mediated Signaling Pathway, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
抗原
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