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Interleukin 17a 抗体

This 山羊 多克隆 anti-Interleukin 17a antibody specifically detects Interleukin 17a in ELISA, WB 和 Neut. The antibody is reactive with 人 samples.
产品编号 ABIN465126
发货至: 中国
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Quick Overview for Interleukin 17a 抗体 (ABIN465126)

抗原

See all Interleukin 17a (IL17A) 抗体
Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This Interleukin 17a antibody is un-conjugated

应用范围

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ELISA, Western Blotting (WB), Neutralization (Neut)
  • 特异性

    Human IL-17A

    纯化方法

    Immunoaffinity purified

    免疫原

    E.coli derived recombinant Human IL-17A.

    Type of Immunogen: Cell extract
  • 应用备注

    Approved: ELISA (0.5 - 2 μg/mL), Neut (0.43 - 0.65 μg/mL), WB

    Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.125 μg/mL-0.25 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-17A (50.00 ng/mL), a concentration of 0.43-0.65 μg/mL of this antibody is required. ELISA: To detect hIL-17A by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-17A (LS-C104417) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-17A. Western Blot: To detect hIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17A is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitute in sterile water

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS,  pH 7.2

    注意事项

    Avoid freeze/thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原

    Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

    别名

    IL17A / IL17

    背景

    Name/Gene ID: IL17A
    Family: Interleukin

    Synonyms: IL17A, CTLA-8, CTLA8, Interleukin-17A, Interleukin 17A, IL-17, IL-17A, IL17

    基因ID

    3605

    UniProt

    Q16552
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