Interferon gamma 抗体 (Biotin)
Quick Overview for Interferon gamma 抗体 (Biotin) (ABIN464925)
抗原
See all Interferon gamma (IFNG) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Rat IFN-gamma
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纯化方法
- Immunoaffinity purified
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免疫原
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E.coli derived recombinant Rat IFN-gamma.
Type of Immunogen: Cell extract
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应用备注
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Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)
Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Rat IFN-gamma by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IFN-gamma. To detect Rat IFN-gamma by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Rat IFN-gamma (LS-C104323) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IFN-gamma. Western Blot: To detect Rat IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IFN-gamma is 1.5-3 ng/lane, under either reducing or non-reducing conditions. -
说明
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Target Species of Antibody: Rat
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile PBS containing 0.1 % BSA
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, pH 7.2
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注意事项
- Avoid freeze/thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Interferon gamma (IFNG)
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别名
- IFN Gamma / Interferon Gamma
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背景
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Name/Gene ID: IFNG
Family: Interferon
Synonyms: IFNG, IFG, Immune interferon, IFI, Interferon gamma, IFN-gamma, Interferon, gamma -
基因ID
- 3458
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UniProt
- P01579
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途径
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
抗原
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