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Galectin 3 抗体

The 兔 多克隆 anti-Galectin 3 antibody is suitable to detect Galectin 3 in samples from 人. It has been validated for WB 和 ELISA.
产品编号 ABIN464873
发货至: 中国
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Quick Overview for Galectin 3 抗体 (ABIN464873)

抗原

See all Galectin 3 (LGALS3) 抗体
Galectin 3 (LGALS3)

适用

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克隆类型

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多克隆

标记

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This Galectin 3 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA
  • 特异性

    Human Galectin-3

    纯化方法

    Immunoaffinity purified

    免疫原

    E.coli derived recombinant Human Galectin-3.

    Type of Immunogen: Cell extract
  • 应用备注

    Approved: ELISA, WB (0.1 - 0.2 μg/mL)

    Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas infiltrating ductal adenocarcinoma tissue. The recommended concentration is 31.25 ng/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a  pH 6 sodium citrate buffer. Optimal concentrations and conditions may vary. ELISA: To detect hGalectin-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Galectin-3 (LS-C104371) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hGalectin-3. Western Blot: To detect hGalectin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGalectin-3 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitute with sterile water to a concentration of 0.1-1.0 mg/mL.

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS,  pH 7.2

    注意事项

    Avoid freeze/thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原

    Galectin 3 (LGALS3)

    别名

    MAC-2 / Galectin 3

    物质类

    Chemical

    背景

    Name/Gene ID: LGALS3

    Synonyms: LGALS3, 35 kd lectin, 35 kDa lectin, CBP35, GAL3, Galactoside-binding protein, GALIG, IgE-binding protein, Laminin-binding protein, Gal-3, GALBP, MAC-2, Mac-2 antigen, L31, MAC2, CBP 35, Galactose-specific lectin 3, Galectin-3, L-31, Lectin L-29

    基因ID

    3958

    UniProt

    P17931

    途径

    RTK signaling
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