Caspase 7 抗体
Quick Overview for Caspase 7 抗体 (ABIN462356)
抗原
See all Caspase 7 (CASP7) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
品牌
- IHC-plus™
-
特异性
- Detects an ~37 kD protein, corresponding to the apparent molecular mass of the caspase-7 on SDS-PAGE immunoblots, in samples from human, mouse, rat, monkey, dog, sheep, rabbit, pig and hamster origins. This antibody recognizes the unprocessed pro-caspase-7 (p35), caspase-7 lacking the short amino- terminal pro domain (32 kD) and the fully processed caspase-7 (p19) in apoptotic cell extracts.
-
纯化方法
- Protein G purified
-
免疫原
-
Recombinant human caspase-7 full length protein.
Type of Immunogen: Recombinant protein -
亚型
- IgG
-
-
-
-
应用备注
-
Approved: IHC, IHC-P (10 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. -
说明
-
Target Species of Antibody: Human
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- PBS (10 mM sodium phosphate, pH 7.2, 50 mM sodium chloride, 0.1 mM PMSF). Sourced in Ascites.
-
储存液
- PMSF
-
注意事项
- avoid freeze thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
-
-
- Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))
-
别名
- CASP7 / Caspase 7
-
背景
-
Name/Gene ID: CASP7
Subfamily: Cysteine C14
Family: Protease
Synonyms: CASP7, Apoptotic protease Mch-3, Caspase 7, Caspase-7, ICE-LAP3, LICE2, MCH3, ICE-like apoptotic protease 3, CASP-7, CMH-1 -
基因ID
- 840
-
UniProt
- P55210
-
途径
- Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
抗原
-