FOXO3 抗体 (pThr24, Thr32)
Quick Overview for FOXO3 抗体 (pThr24, Thr32) (ABIN461993)
抗原
See all FOXO3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr24, Thr32
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品牌
- IHC-plus™
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特异性
- Detects endogenous levels of FKHR (FKHRL1) only when activated by phosphorylation at Thr24 of FKHR or Thr32 of FKHRL1. Also reacts with phosphorylated AFX at Thr28. Does not react with FKHR family members phosphorylated at other sites. Species cross-reactivity: Human, mouse, rat.
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纯化方法
- Protein A purified
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免疫原
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Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr24 of human FKHR.
Type of Immunogen: Synthetic phospho-peptide -
亚型
- IgG
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应用备注
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Approved: ELISA, IHC, IHC-P (1:100), IP (1:50), WB (1:1000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:100. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.
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储存液
- Without preservative
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- FOXO3 (Forkhead Box O3 (FOXO3))
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别名
- FOXO3 / FOXO3A
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背景
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Name/Gene ID: FOXO3
Family: Transcription factor
Synonyms: FOXO3, AF6q21, AF6q21 protein, Forkhead box O3, Forkhead box O3A, FOXO3A, FKHRL1P2, Forkhead box protein O3, FOXO2, FKHRL1 -
基因ID
- 2309
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UniProt
- O43524
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途径
- Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis
抗原
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