PLG 抗体 (Biotin)
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北京 101111
Quick Overview for PLG 抗体 (Biotin) (ABIN459099)
抗原
See all PLG 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Rabbit anti Human Plasminogen, conjugated with Biotin
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特异性
- Biotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to plasminogen from Human plasma.
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交叉反应 (详细)
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
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产品特性
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
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纯化方法
- Purified IgG fraction from antiserum by ammonium sulphate precipitation and ion exchange chromatography.
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免疫原
- Plasminogen isolated and purified from human plasma. Freund's complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1.0 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
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缓冲液
- Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- PLG (Plasminogen (PLG))
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别名
- Plasminogen
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UniProt
- P00747
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途径
- Complement System, Lipid Metabolism
抗原
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