L-Alanine Dehydrogenase 抗体
适用: Bacillus subtilis
ELISA, WB, IF
宿主: 兔
Polyclonal
unconjugated
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- 抗原
- L-Alanine Dehydrogenase
- 适用
- Bacillus subtilis
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- ELISA, Western Blotting (WB), Immunofluorescence (IF)
- 特异性
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
- 产品特性
- Affinity purified antibodies of polyclonal rabbit antiserum to L-alanine dehydrogenase from Bacillus subtilis
- 纯化方法
- Specific polyclonal antibody fractions were prepared by solid phase affinity chromatography.
- 免疫原
- L-Alanine dehydrogenase isolated and purified from Bacillus subtilis. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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- 应用备注
- This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- PAb concentration 1 mg/0.5ml. No foreign proteins added.
- 缓冲液
- Purified hyperimmune rabbit IgG antibodies lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2), stabilized with dextran.
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilised PAb fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is rec onstituted by adding 0.5 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- 抗原
- L-Alanine Dehydrogenase
- 背景
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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