L-Alanine Dehydrogenase 抗体 (Biotin)
适用: Bacillus subtilis
ELISA, WB, IF
宿主: 兔
Polyclonal
Biotin
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- 抗原
- L-Alanine Dehydrogenase
- 适用
- Bacillus subtilis
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- Biotin
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应用范围
- ELISA, Western Blotting (WB), Immunofluorescence (IF)
- 特异性
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
- 产品特性
- Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to L-alanine dehydrogenase from Bacillus subtilis
- 纯化方法
- The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
- 免疫原
- L-Alanine dehydrogenase isolated and purified from Bacillus subtilis. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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- 应用备注
- This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- IgG protein concentration 10 mg/ml. Biotin/ IgG protein molar ratio (B/P) approximately 5.4. No foreign proteins added.
- 缓冲液
- Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- 抗原
- L-Alanine Dehydrogenase
- 别名
- SCI51.13c antibody, CMS2925 antibody, L-alanine dehydrogenase antibody, l-alanine dehydrogenase antibody, SCO1773 antibody, Mmcs_2104 antibody, Mmar10_1058 antibody, Mmc1_0055 antibody, Acel_1288 antibody, Noca_2488 antibody, ald antibody, Toce_1453 antibody, Ilyop_0407 antibody, Palpr_1204 antibody, Ftrac_3618 antibody, Ocepr_1994 antibody, Sulku_1314 antibody, Tmar_1146 antibody, Intca_1842 antibody, Bache_1992 antibody, Isop_1447 antibody, Despr_2708 antibody, Plabr_0310 antibody
- 背景
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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