Erythrocytes 抗体
适用: 绵羊
ScA
宿主: 兔
Polyclonal
unconjugated
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- 抗原
- Erythrocytes
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适用
- 绵羊
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Screening Assay (ScA)
- 特异性
- Homologous proteins of different species frequently share antigenic determinants. has not been tested.
- 产品特性
- IgG fraction of polyclonal rabbit antiserum to sheep erythrocytes
- 纯化方法
- Adsorption: No adsorption required. Cross-reactivity Homologous proteins of different species frequently share antigenic determinants. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
- 免疫原
- Washed whole erythrocytes were prepared from fresh blood and used for intravenous immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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anti-Erythrocytes antibody
适用: 人 H, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Erythrocytes antibody适用: 大鼠 FACS 宿主: 小鼠 Monoclonal OX-83 unconjugated
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- 应用备注
- To sensitize cells in haemolytic complement assays and for screening of T and T receptor-bearing lymphocytes.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- IgG protein concentration 10 mg/ml. No foreign proteins added. Antibody titre: Haemolytic titre, at which 50% lysis occurs following mixing of equal volumes of antiserum, guinea pig complement (at a 1:60 dilution) and 5x10 8 RBC/ml, not less than 1:3,000
- 缓冲液
- Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.
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- 抗原
- Erythrocytes
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