Homologous proteins of different species frequently share antigenic determinants. has not been tested.
产品特性
Polyclonal rabbit antiserum to bovine erythrocytes
纯化方法
Adsorption: No adsorption required. Cross-reactivity Homologous proteins of different species frequently share antigenic determinants.
免疫原
Washed whole erythrocytes were prepared from fresh blood and used for intravenous immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.
To sensitize cells in haemolytic complement assays and for screening of T and T receptor-bearing lymphocytes.
限制
仅限研究用
状态
Lyophilized
浓度
Total protein and IgG concentrations in the antiserum are comparable to those in pooled rabbit serum. No foreign proteins added. Antibody titre: Haemolytic titre, at which 50% lysis occurs following mixing of equal volumes of antiserum, guinea pig complem
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Recon stitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.