Total Serum Proteins 抗体 (FITC)
适用: 大鼠
ISt
宿主: 山羊
Polyclonal
FITC
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- 抗原
- Total Serum Proteins
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适用
- 大鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- FITC
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应用范围
- Immunostaining (ISt)
- 特异性
- Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.
- 产品特性
- Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal goat antiserum to rat total serum proteins
- 纯化方法
- Adsorption: No adsorption required. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
- 免疫原
- Pooled whole serum and selected protein fractions isolated from pooled rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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- 应用备注
- Direct fluorescent staining of rat serum proteins at the cellular and subcellular level of appropriately treated cell and tissue substrates. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- IgG protein concentration 10 mg/ml. Fluorochrome/IgG protein molar ratio (F/P) is approximately 1.8. No foreign proteins added.
- 缓冲液
- FITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile di stilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
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- 抗原
- Total Serum Proteins
- 背景
- Tested in immunoelectrophoresis and double radial immunodiffusion against pooled serum precipi-tation of at least 12 individual proteins have been observed
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