Total Serum Proteins 抗体 (FITC)
The 山羊 多克隆 anti-Total Serum Proteins antibody is suitable to detect Total Serum Proteins in samples from 兔. It has been validated for ISt.
产品编号 ABIN458585
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Total Serum Proteins 抗体 (FITC) (ABIN458585)
抗原
Total Serum Proteins
适用
兔
宿主
山羊
克隆类型
多克隆
标记
FITC
应用范围
Immunostaining (ISt)
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特异性
- Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.
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产品特性
- Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal goat antiserum to rabbit total serum proteins
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纯化方法
- Adsorption: No adsorption required. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
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免疫原
- Pooled whole serum and selected protein fractions isolated from pooled rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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应用备注
- Direct fluorescent staining of rabbit serum proteins at the cellular and subcellular level of appropriately treated cell and tissue substrates. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.
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限制
- 仅限研究用
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状态
- Lyophilized
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浓度
- IgG protein concentration 10 mg/ml. Fluorochrome/IgG protein molar ratio (F/P) is approximately 1.6. No foreign proteins added.
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缓冲液
- FITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
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储存液
- Without preservative
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile di stilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C , not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
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- Total Serum Proteins
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背景
- Tested in immunoelectrophoresis and double radial immunodiffusion against pooled serum precipitation of at least 14 individual proteins have been observed
抗原
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