Transferrin 抗体
Quick Overview for Transferrin 抗体 (ABIN458572)
抗原
See all Transferrin (TF) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.
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产品特性
- Purified IgG fraction of polyclonal rabbit antiserum to swine transferrin
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纯化方法
- Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong activity are selected for fractionation by salt-precipitation and purification of the IgG (7S) fraction by DEAE-chromatography.
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免疫原
- Purified transferrin isolated from pooled swine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-Transferrin (TF) (AA 361-683) antibody
TF 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) antibodyTF 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) antibodyTF 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) (AA 22-176) antibodyTF 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) (AA 551-650) antibodyTF 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1C2 unconjugated
anti-Transferrin (TF) antibodyTF 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) antibodyTF 适用: 人 WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal 1F10 unconjugated
anti-Transferrin (TF) (AA 284-575) antibodyTF 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) antibodyTF 适用: 人 WB, IHC (p), IF 宿主: 小鼠 Monoclonal 9B3 unconjugated
anti-Transferrin (TF) (AA 432-466) antibodyTF 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB55980 unconjugated
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应用备注
- As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration . Typical working dilutions in histochemistry are usually between 1:50 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5,000.
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限制
- 仅限研究用
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状态
- Lyophilized
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浓度
- IgG protein concentration 10 mg/ml. No foreign proteins added. Antibody titre: Precipitin titre 1:32 when tested against pooled normal swine serum in agar-block immuno-diffusion titration.
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缓冲液
- Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
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储存液
- Without preservative
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.
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- Transferrin (TF)
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别名
- Transferrin
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背景
- The reactivity of the antiserum is restricted to transferrin as tested in immunoelectrophoresis and radial immunodiffusion. A characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified transferrin
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途径
- Transition Metal Ion Homeostasis
抗原
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