Transferrin 抗体 (FITC)
Quick Overview for Transferrin 抗体 (FITC) (ABIN458564)
抗原
See all Transferrin (TF) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.
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产品特性
- Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal rabbit antiserum to swine transferrin
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纯化方法
- Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
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免疫原
- Purified transferrin isolated from pooled swine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-Transferrin (TF) (AA 361-683) antibody
TF 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) antibodyTF 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) antibodyTF 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) (AA 22-176) antibodyTF 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) (AA 551-650) antibodyTF 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1C2 unconjugated
anti-Transferrin (TF) antibodyTF 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) antibodyTF 适用: 人 WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal 1F10 unconjugated
anti-Transferrin (TF) (AA 284-575) antibodyTF 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) antibodyTF 适用: 人 WB, IHC (p), IF 宿主: 小鼠 Monoclonal 9B3 unconjugated
anti-Transferrin (TF) (AA 432-466) antibodyTF 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB55980 unconjugated
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应用备注
- In immunocytochemical and immunohistochemical assays to identify and measure transferrin at the cellular and subcellular level by fluorescent staining of appropriately treated cell and tissue substrates. In non-isotopic assay methodology (e.g. ELISA and Western blotting) to identify and measure transferrin in swine serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.
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限制
- 仅限研究用
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状态
- Lyophilized
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浓度
- IgG protein concentration 10 mg/ml. Fluorochrome/IgG protein molar ratio (F/P) is approximately 1.5. No foreign proteins added.
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缓冲液
- FITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
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储存液
- Without preservative
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized immunoconjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.
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- Transferrin (TF)
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别名
- Transferrin
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背景
- The reactivity of the antiserum is restricted to transferrin as tested in immunoelectrophoresis and radial immunodiffusion. A characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified transferrin
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途径
- Transition Metal Ion Homeostasis
抗原
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