Complement C3c 抗体
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北京 101111
Quick Overview for Complement C3c 抗体 (ABIN458242)
抗原
适用
宿主
克隆类型
标记
应用范围
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特异性
- The antiserum does not cross-react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has not been tested in detail.
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产品特性
- Purified IgG fraction of polyclonal swine antiserum to C3c fragment of human complement factor C3
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纯化方法
- Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. The IgG (7S) fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immunodiffusion.
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免疫原
- C3c is the major fragment resulting from C3 cleavage by C3 convertase and factor I. It is composed of an intact beta chain bound to two fragments of the alpha chain. C3c is isolated and purified from pooled normal human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-Complement Component C3c (C3C) (AA 1521-1649) antibody
C3C 适用: 人 ELISA, WB, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 4C6A8 unconjugated
anti-Complement Component C3c (C3C) (AA 1521-1649) antibodyC3C 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 8B5H2 unconjugated
anti-Complement Component C3c (C3C) antibodyC3C 适用: 人 ELISA, IHC, IHC (fro), IHC (p) 宿主: 绵羊 Polyclonal unconjugated
anti-Complement Component C3c (C3C) antibodyC3C 适用: 人 IHC (p), ID 宿主: 兔 Polyclonal unconjugated
anti-Complement Component C3c (C3C) antibodyC3C 适用: 人 IHC (p), ID 宿主: 山羊 Polyclonal unconjugated
anti-Complement Component C3c (C3C) (AA 1051-1200) antibodyC3C 适用: 人, 大鼠, Pig ELISA, WB, ICC, IF (p), IF (cc), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
- As unlabelled primary or secondary antibody for the detection of C3c at the cellular and subcellular level by immunofluorescence and immunoenzyme assay methods, for the production of immunoconjugates with a selected marker, to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier, as catching or detecting antibody in non-isotopic methodology and solid phase immunochemistry. Determinations of individual complement components can be useful in defining the exact location of a defect. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling technique, the optimum working dilution should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5,000.
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限制
- 仅限研究用
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状态
- Lyophilized
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浓度
- IgG concentration 10 mg/ml. No foreign proteins added. Antibody titre: Precipitin titre not lees than 1:16 when tested against normal human serum in agar block immunodiffusion titration.
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缓冲液
- Purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2)
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储存液
- Without preservative
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储存条件
- 4 °C
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储存方法
- The IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile di stilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, a nd preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- Complement C3c (C3C) (Complement Component C3c (C3C))
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别名
- C3c Fragment of Complement Factor C3
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背景
- In immunoelectrophoresis against fresh human serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c cab also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of human serum or plasma
抗原
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