Complement C3c 抗体 (FITC)
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北京 101111
Quick Overview for Complement C3c 抗体 (FITC) (ABIN458597)
抗原
适用
宿主
克隆类型
标记
应用范围
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原理
- Goat anti Rabbit C3c, conjugated with FITC
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特异性
- Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to C3c fragment of Rabbit complement factor C3.
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交叉反应 （详细）
- The antiSerum does not cross-react with any other component of Rabbit plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
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产品特性
- In immunoelectrophoresis against fresh rabbit serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c can also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of rabbit serum or plasma. The fluorescent immunoconjugate to rabbit C3c is used to determine the presence and pattern of C3 in tissue lesions using immunohistochemical staining techniques. Locally deposited immune complexes in tissue usually contain complement, pointing to activation of the classical pathway. Complement activation in vivo implies active disease and may contribute to the elicitation of the pathogenesis and he extent of tissue destruction. Sometimes the diagnosis can be based on directly on laboratory findings. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
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纯化方法
- Purified IgG fraction from antiserum
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免疫原
- C3c is the major fragment resulting from C3 cleavage by C3 convertase and factor I. It is composed of an intact beta chain bound to two fragments of the alpha chain. C3c is isolated and purified from pooled normal rabbit serum. Freund's complete adjuvant is used in the first step of the immunization procedure. Freund's complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-Complement Component C3c (C3C) (AA 1521-1649) antibody
C3C 适用: 人 ELISA, WB, ICC, IHC, FACS 宿主: 小鼠 Monoclonal 4C6A8 unconjugated
anti-Complement Component C3c (C3C) (AA 1521-1649) antibodyC3C 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 8B5H2 unconjugated
anti-Complement Component C3c (C3C) antibodyC3C 适用: 人 ELISA, IHC, IHC (fro), IHC (p) 宿主: 绵羊 Polyclonal unconjugated
anti-Complement Component C3c (C3C) antibodyC3C 适用: 人 IHC (p), ID 宿主: 山羊 Polyclonal unconjugated
anti-Complement Component C3c (C3C) antibodyC3C 适用: 人 IHC (p), ID 宿主: 兔 Polyclonal unconjugated
anti-Complement Component C3c (C3C) (AA 1051-1200) antibodyC3C 适用: 人 WB, IHC (fro), IHC (p), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
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应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
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缓冲液
- Fluorochrome-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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: "Vaccine Platform Comparison: Protective Efficacy against Lethal Marburg Virus Challenge in the Hamster Model." in: International journal of molecular sciences, Vol. 25, Issue 15, (2024) (PubMed).
: "Rapid protection of nonhuman primates against Marburg virus disease using a single low-dose VSV-based vaccine." in: EBioMedicine, Vol. 89, pp. 104463, (2023) (PubMed).
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- Complement C3c (C3C) (Complement Component C3c (C3C))
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别名
- C3c Fragment of Complement Factor C3
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UniProt
- P12247
抗原
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