Complement C3c 抗体
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- 抗原 See all Complement C3c (C3C) products
- Complement C3c (C3C) (Complement Component C3c (C3C))
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适用
- 人
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宿主
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猪
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克隆类型
- 多克隆
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标记
- This Complement C3c antibody is un-conjugated
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应用范围
- Immunoelectrophoresis (IEP)
- 特异性
- Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.
- 产品特性
- Precipitating polyclonal swine antiserum to C3c subunit of human complement C3
- 纯化方法
- Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
- 免疫原
- C3c is the major fragment resulting from the C3 cleavage by C3 convertase and factor i. It is composed of an intact beta chain bound to two fragments of the alpha chain. The protein is isolated and purified from pooled normal human serum by precipitation techniques, followed by chromatographical methods. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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anti-Complement Component C3c (C3C) (AA 1521-1649) antibody
C3C 适用: 人 ELISA, IHC, WB, ICC, FACS 宿主: 小鼠 Monoclonal 8B5H2 unconjugated
anti-Complement Component C3c (C3C) antibodyC3C 适用: 人 IHC (p), ID 宿主: 兔 Polyclonal unconjugated
anti-Complement Component C3c (C3C) antibodyC3C 适用: 人 ELISA, IHC, IHC (fro), IHC (p) 宿主: 绵羊 Polyclonal unconjugated
anti-Complement Component C3c (C3C) antibodyC3C 适用: 人 IHC (p), ID 宿主: 山羊 Polyclonal unconjugated
anti-Complement Component C3c (C3C) (AA 1051-1200) antibodyC3C 适用: 人, 大鼠, Pig ELISA, WB, ICC, IHC (fro), IHC (p), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- In precipitating techniques as immunoelectrophoresis and single and double radial immuno-diffusion (Mancini, Ouchterlony) to identify the presence of complement C3c or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included. Determinations of individual complement components can be very useful in defining the exact location of a defect.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled swine serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:32 when tested against normal human plasma in agar-block immunodiffusio
- 缓冲液
- Delipidated, heat inactivated, lyophilized, stable whole serum.
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.
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- 抗原
- Complement C3c (C3C) (Complement Component C3c (C3C))
- 别名
- C3c Subunit of Complement C3 (C3C 产品)
- 背景
- In immunoelectrophoresis against fresh human serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c can also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of human serum or plasma
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