Complement C3b 抗体
The 兔 多克隆 anti-Complement C3b antibody is suitable to detect Complement C3b in samples from 人. It has been validated for IEP 和 RID.
产品编号 ABIN458141
发货至:
中国
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中国
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北京 101111
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Quick Overview for Complement C3b 抗体 (ABIN458141)
抗原
See all Complement C3b (C3b) 抗体
Complement C3b (C3b)
(Complement C3 beta Chain (C3b))
适用
人
宿主
兔
克隆类型
多克隆
标记
This Complement C3b antibody is un-conjugated
应用范围
Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)
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原理
- Rabbit anti Human C3b
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特异性
- Precipitin titre not less than 1:16 when tested anti normal Human plasma in agar-block immunodiffusion titRation.
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交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Crossreactivity of this antiSerum has not been tested in detail.
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产品特性
- In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against human plasma a single precipitin line is obtained in the beta-1 region and shows a reaction of full identity with the purified immunogen. The antiserum does not react with any other protein component of human serum or plasma. In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of complement C3b or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included. Determinations of individual complement components can be very useful in defining the exact location of a defect.
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纯化方法
- Delipidated, heat inactivated, lyophilized, stable whole antiserum
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免疫原
- C3b (C3c+C3d) has a molecular weight of 170,000. The protein is isolated and purified from pooled normal human serum by precipitation techniques, followed by chromatographical methods. Freund's complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-Complement C3 beta Chain (C3b) (AA 581-667) antibody
C3b 适用: 人 WB, IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Precipitation assays. In immunoelectrophoresis use 2 μL human plasma or equivalent against 120 μL antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μL antiserum in 3 mm diameter center well and 2 μL plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single radial immunodiffusion use 1 percent antiserum in the gel.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
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缓冲液
- Delipidated, heat inactivated, lyophilized, stable whole serum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum. No foreign proteins added.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
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- Complement C3b (C3b) (Complement C3 beta Chain (C3b))
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别名
- C3b Subunit of Complement C3
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UniProt
- P01024
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途径
- Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling
抗原
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