PROS1 抗体
Quick Overview for PROS1 抗体 (ABIN458052)
抗原
See all PROS1 (PROS) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- The antiserum does not cross react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has not been tested in detail.
-
产品特性
- Precipitating polyclonal goat antiserum to human protein S
-
纯化方法
- Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
-
免疫原
- Protein S is a vitamin K dependent plasma glycoprotein (MW 69,000) which belongs to a group of coagulation inhibitors consisting itself of proteinases. It circulates in the blood in a free active form and in an inactive form bound to C4b binding protein. Protein S is also released by thrombin stimulation from platelet alpha-granules. Protein S acts a s a cofactor for activated protein C (pCa) enhancing the cleavage of factors Va and VIIIa, producing anticoagulation by decreasing the conversion of prothrombin to thrombin. It also stimulates the fibrinolytic system. Protein S looses much of its coagulant cofactor activity after cleavage by thrombin, a process which is inhibited by thrombomodulin in the presence of free calcium ions. Plasma concentration of protein S may be significantly reduced below normal adult levels (2.5 mg/ml) in patients with hereditary deficiency of protein S or suffering a combination of deep vein thrombosis, superficial thrombophlebitis and pulmonary embolism. Substances such as bacterial endotoxins, tumour necrosis factor and immune mediator interleukin 1 also reduce the protein S level in plasma. Oral contraceptives also depress pS activity. A reduction in plasma protein S during pregnancy usually returns to normal after parturition. Freund’s complete adjuvant is used in the first step of the immunization procedure.
-
亚型
- IgG
-
-
anti-Protein S (PROS) (AA 481-667) antibody
PROS 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein S (PROS) (N-Term) antibodyPROS 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein S (PROS) (AA 42-676) antibodyPROS 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein S (PROS) (AA 1-676) antibodyPROS 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Protein S (PROS) (AA 245-295) antibodyPROS 适用: 人, 大鼠, 小鼠 WB, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein S (PROS) antibodyPROS 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein S (PROS) (AA 419-516) antibodyPROS 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Protein S (PROS) (AA 251-300) antibodyPROS 适用: 人, 大鼠, 小鼠, 犬, 马, Cow, Bat, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein S (PROS) (AA 405-676) antibodyPROS 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Protein S (PROS) (AA 416-646) antibodyPROS 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- In precipitating techniques as immunoelectrophoresis, single or double radial immunodiffusion, electro-immunodiffusion, immunonepholometry as well as solid phase and neutralization reactions. As catching antibody in ELISA, to prepare an adsorbent for immunoaffinity purification of protein S. Plasma samples and all assay components must contain EDTA to stabilize the protein.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
浓度
- Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Antibody titre: Precipitin titre 1:32 when tested against pooled normal human plasma in agar-block immunodiffusion titrat
-
缓冲液
- Delipidated, heat inactivated, lyophilized, stable whole antiserum
-
储存液
- Without preservative
-
储存条件
- 4 °C/-20 °C
-
储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged stora ge at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.
-
-
- PROS1 (PROS) (Protein S (PROS))
-
别名
- Proteins
-
背景
- The defined antibody specificity is restricted to protein S as tested at the level of sensitivity of immuno-precipitation techniques and ELISA techniques. A single precipitin line is obtained in immunoelectrophoresis, bidimensional electrophoresis and double radial immunodiffusion (Ouchterlony) against normal plasma, which shows a reaction of identity with the precipitated purified protein S. The antiserum also reacts with the protein S component in complexes with protein C or with C4b binding protein. No reaction is obtained with any other plasma protein
抗原
-
