CFP 抗体 (FITC)
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北京 101111
Quick Overview for CFP 抗体 (FITC) (ABIN458013)
抗原
See all CFP 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Goat anti Human properdin, conjugated with FITC
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特异性
- Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to Human properdin
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交叉反应 （详细）
- The antiSerum does not cross-react with any other Human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
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产品特性
- In immunoelectrophoresis against human serum a single precipitin line is obtained. The antiserum does not react with any other protein component of human serum or plasma. As reagent for the direct detection of properdin in human cells, tissues and body fluids in immunofluorescence, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA). This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
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纯化方法
- Purified IgG fraction from antiserum
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免疫原
- Properdin is a factor of the alternative complement activation pathway. It has a molecular weight of 222,000 and consists of four peptide chains. Properdin is stabilizing the C3 convertase C3bBb. The mean concentration in plasma is 20 mg/ml. The antigen has been isolated form pooled human plasma. Freund's complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-Complement Factor P (CFP) (AA 190-469) antibody
CFP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Complement Factor P (CFP) (C-Term) antibodyCFP 适用: 人, 小鼠, 大鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Complement Factor P (CFP) (AA 401-469) antibodyCFP 适用: 人 WB, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Complement Factor P (CFP) (AA 197-223) antibodyCFP 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB22181 unconjugated
anti-Complement Factor P (CFP) (AA 35-84) antibodyCFP 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Complement Factor P (CFP) (AA 315-469) antibodyCFP 适用: 人 WB, FACS 宿主: 兔 Polyclonal unconjugated
anti-Complement Factor P (CFP) (AA 54-217) antibodyCFP 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Complement Factor P (CFP) (AA 65-190) antibodyCFP 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Complement Factor P (CFP) (AA 197-223) antibodyCFP 适用: 人 WB, FACS, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence. The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- Fluorochrome-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.
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储存液
- Without preservative
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- CFP (Complement Factor P (CFP))
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别名
- Properdin
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UniProt
- P27918
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途径
- Complement System
抗原
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