CFP 抗体 (FITC)
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北京 101111
Quick Overview for CFP 抗体 (FITC) (ABIN458013)
抗原
See all CFP 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.
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产品特性
- Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal goat antiserum to human properdin
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纯化方法
- Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
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免疫原
- Properdin is a factor of the alternative complement activation pathway. It has a molecular weight of 222,000 and consists of four peptide chains. Properdin is stabilizing the C3 convertase C3bBb. The mean concentration in plasma is 20 mg/ml. The antigen has been isolated form pooled human plasma. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-Complement Factor P (CFP) (AA 190-469) antibody
CFP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Complement Factor P (CFP) (AA 401-469) antibodyCFP 适用: 人, 小鼠 WB, ELISA, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Complement Factor P (CFP) (AA 315-469) antibodyCFP 适用: 人 WB, IHC, IP, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Complement Factor P (CFP) (Internal Region) antibodyCFP 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Complement Factor P (CFP) (AA 35-84) antibodyCFP 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Complement Factor P (CFP) (AA 54-217) antibodyCFP 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Complement Factor P (CFP) (AA 65-190) antibodyCFP 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Complement Factor P (CFP) (AA 197-223) antibodyCFP 适用: 人 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Complement Factor P (CFP) (AA 310-459) antibodyCFP 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Complement Factor P (CFP) (AA 1-469) antibodyCFP 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- As reagent for the direct detection of properdin in human cells, tissues and body fluids in immuno-fluorescence, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA). This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:60.
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限制
- 仅限研究用
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状态
- Lyophilized
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浓度
- IgG protein concentration 10.7 mg/ml. Fluorescein/IgG protein molar ratio (F/P) approximately 1.3. No foreign proteins added.
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缓冲液
- Fluorochrome-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2)
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储存液
- Without preservative
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储存条件
- 4 °C
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- CFP (Complement Factor P (CFP))
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别名
- Properdin
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背景
- Tested in immunoelectrophoresis against human serum a single precipitin line is obtained. The antiserum does not react with any other protein component of human serum or plasma
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途径
- Complement System
抗原
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