C5 抗体 (FITC)
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北京 101111
Quick Overview for C5 抗体 (FITC) (ABIN458008)
抗原
See all C5 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Goat anti Human C5, conjugated with FITC
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特异性
- Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal goat antiserum to C5 fragment of human complement
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交叉反应 （详细）
- The antiSerum does not cross-react with any other component of Human plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
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产品特性
- Tested in immunoelectrophoresis and double radial immunodiffusion against normal human serum, a single precipitin line is obtained which gives a reaction of full identity with the isolated immunogen. The product does not react with any other proteins component of human serum or plasma. The fluorescent immunoconjugate to human C5 is used to determine the presence and pattern using immunohistochemical staining techniques. Determinations of individual C components can be very useful in defining the exact location of a defect. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
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纯化方法
- Purified IgG fraction from antiserum
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免疫原
- C5 is a component of the complement system. It has a molecular weight of 190,000. The activated protein forms with the complement factors C6-C9 the membrane attack-complex. C5 is isolated and purified from pooled normal human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-Complement Component 5 (C5) (AA 701-755) antibody
C5 适用: 小鼠 WB, IF (p), IF (cc), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 5 (C5) (N-Term) antibodyC5 适用: 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 5 (C5) (AA 253-281), (N-Term) antibodyC5 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB21866 unconjugated
anti-Complement Component 5 (C5) (AA 253-281) antibodyC5 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 5 (C5) (C-Term) antibodyC5 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 5 (C5) (AA 1-77) antibodyC5 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 5 (C5) (AA 1550-1661) antibodyC5 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
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缓冲液
- Fluorochrome-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
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- C5 (Complement Component 5 (C5))
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别名
- C5 Fragment of Complement
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背景
- Human complement C5
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UniProt
- P01031
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途径
- Complement System, Carbohydrate Homeostasis
抗原
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