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CYB5R3 抗体 (C-Term)

Name: CYB5R3 抗体 (C-Term)
SKU: ABIN453978
Availability: OutOfStock

CYB5R3 适用: 小鼠, 大鼠, 犬, Cow WB, EIA 宿主: 山羊 Polyclonal unconjugated

Image no. 1 for anti-Cytochrome B5 Reductase 3 (CYB5R3) (C-Term) antibody (ABIN453978) (CYB5R3 抗体 (C-Term))

1 image

(3 references)

产品编号 ABIN453978

发货至: 中国

Datasheet as PDF

抗原 See all CYB5R3 抗体

CYB5R3 (Cytochrome B5 Reductase 3 (CYB5R3))
抗原表位
8

6

5

3

1

1

1

1

1

1

1

1

1

1

1
C-Term
适用
48

22

17

3

3

3

2

1

1

1
小鼠, 大鼠, 犬, Cow
宿主
47

7

3
山羊
Compatible Secondaries
克隆类型
52

5
多克隆
标记
29

6

5

3

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CYB5R3 antibody is un-conjugated
应用范围
46

24

19

14

6

5

4

4

4

4

4

2

2
Western Blotting (WB), Enzyme Immunoassay (EIA)

特异性

This antibody recognizes CYB5R3.

交叉反应（详细）

Species reactivity (expected):Rat, Canine (Dog) and Cow.
Species reactivity (tested):Mouse.

纯化方法

Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

免疫原

Peptide with sequence PNLERVGHPKERC, from the C-Terminus of the protein sequence according to NP_084063.1.

Top Product

Discover our top product CYB5R3 Primary Antibody
anti-Cytochrome B5 Reductase 3 (CYB5R3) (AA 1-301) antibody
CYB5R3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-Cytochrome B5 Reductase 3 (CYB5R3) (AA 1-301) antibody
CYB5R3 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome B5 Reductase 3 (CYB5R3) (AA 1-301) antibody
CYB5R3 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-Cytochrome B5 Reductase 3 (CYB5R3) antibody
CYB5R3 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome B5 Reductase 3 (CYB5R3) (C-Term) antibody
CYB5R3 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome B5 Reductase 3 (CYB5R3) antibody
CYB5R3 适用: 人, 犬, 猴 WB, IHC, FACS 宿主: 小鼠 Monoclonal 2A10 unconjugated

anti-Cytochrome B5 Reductase 3 (CYB5R3) (AA 111-144) antibody
CYB5R3 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal RB49795 unconjugated

anti-Cytochrome B5 Reductase 3 (CYB5R3) (AA 191-240) antibody
CYB5R3 适用: 人, 兔 WB 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome B5 Reductase 3 (CYB5R3) (AA 157-252) antibody
CYB5R3 适用: 人 ELISA 宿主: 小鼠 Monoclonal 2A9 unconjugated

anti-Cytochrome B5 Reductase 3 (CYB5R3) antibody (HRP)
CYB5R3 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal HRP

应用备注

Peptide ELISA: Detection limit dilution 1/32000. Western blot: 0.1-1 μg/mL. Approx 35 kDa band observed in Mouse Liver lysates as well as inMouse and Rat brain lysates (calculated MW of 34.5 kDa according to Mouse NP_084063.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Species Reactivity: Tested: Mouse. Expected from sequence similarity: Rat, Canine (Dog) and Cow.

限制

仅限研究用
浓度

0.5 mg/mL

缓冲液

Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Reed, Jarvis, Phillips, Arlt: "Enhanced DNA adduct formation by benzo[a]pyrene in human liver cells lacking cytochrome P450 oxidoreductase." in: Mutation research, Vol. 852, pp. 503162, (2020) (PubMed).

Reed, Indra, Mrizova, Moserova, Schmeiser, Wolf, Henderson, Stiborova, Phillips, Arlt: "Application of hepatic cytochrome b5/P450 reductase null (HBRN) mice to study the role of cytochrome b5 in the cytochrome P450-mediated bioactivation of the anticancer drug ellipticine." in: Toxicology and applied pharmacology, Vol. 366, pp. 64-74, (2019) (PubMed).

Reed, Mrizova, Barta, Indra, Moserova, Kopka, Schmeiser, Wolf, Henderson, Stiborova, Phillips, Arlt: "Cytochrome b 5 impacts on cytochrome P450-mediated metabolism of benzo[a]pyrene and its DNA adduct formation: studies in hepatic cytochrome b 5 /P450 reductase null (HBRN) mice." in: Archives of toxicology, Vol. 92, Issue 4, pp. 1625-1638, (2019) (PubMed).
抗原

CYB5R3 (Cytochrome B5 Reductase 3 (CYB5R3))

别名

CYB5R3 (CYB5R3 产品)

别名

B5R antibody, DIA1 antibody, Dia1 antibody, Nadhcb5 antibody, RNNADHCB5 antibody, 0610016L08Rik antibody, 2500002N19Rik antibody, C85115 antibody, Dia-1 antibody, WU:AL591952.1-001 antibody, WU:AL591952.1-002 antibody, WU:AL591952.1-003 antibody, WU:Cyb5r3 antibody, fc32h03 antibody, zgc:77071 antibody, wu:fc32h03 antibody, dia1 antibody, CB5R antibody, DDBDRAFT_0218707 antibody, DDBDRAFT_0266821 antibody, DDB_0218707 antibody, DDB_0266821 antibody, cytochrome b5 reductase 3 antibody, cytochrome b5 reductase 3 L homeolog antibody, NADH-cytochrome b5 reductase antibody, CYB5R3 antibody, Cyb5r3 antibody, cyb5r3 antibody, cyb5r3.L antibody, CNM00240 antibody, Tb11.47.0018 antibody, cyb5r1 antibody

背景

CYB5R3 belongs to the flavoprotein pyridine nucleotide cytochrome reductase family and contains 1 FAD-binding FR-type domain. There are two isoforms, NADH-cytochrome b5 reductase 3 membrane bound form and NADH-cytochrome b5 reductase 3 soluble form. Its functions include desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Defects in CYB5R3 are the cause of hereditary methemoglobinemia (HM). There are three forms of this disease: type 1 (HM1) in which the enzyme is only deficient in erythrocytes with a mild cyanosis, type 2 (HM2), in which the enzyme is completely deficient, type 3 (HM3) where the deficiency is seen in all blood cells. Type 2 is a severe form accompanied with mental retardation and neurological impairment.Synonyms: B5R, DIA1, Diaphorase-1, NADH-cytochrome b5 reductase 3

基因ID

1727

NCBI登录号

NP_000389

UniProt

P00387

途径

SARS-CoV-2 Protein Interactome