CYB5R3 抗体 (C-Term)
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Quick Overview for CYB5R3 抗体 (C-Term) (ABIN453978)
抗原
See all CYB5R3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- This antibody recognizes CYB5R3.
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交叉反应 （详细）
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Species reactivity (expected):Rat, Canine (Dog) and Cow.
Species reactivity (tested):Mouse. -
纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence PNLERVGHPKERC, from the C-Terminus of the protein sequence according to NP_084063.1.
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anti-Cytochrome B5 Reductase 3 (CYB5R3) (AA 1-301) antibody
CYB5R3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Cytochrome B5 Reductase 3 (CYB5R3) (AA 1-301) antibodyCYB5R3 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome B5 Reductase 3 (CYB5R3) antibodyKD Validated CYB5R3 适用: 人 WB, FACS, IF (p) 宿主: 兔 Monoclonal 25GB2805 unconjugated Recombinant Antibody
anti-Cytochrome B5 Reductase 3 (CYB5R3) (AA 1-301) antibodyCYB5R3 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Cytochrome B5 Reductase 3 (CYB5R3) antibodyCYB5R3 适用: 人, 大鼠, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome B5 Reductase 3 (CYB5R3) (C-Term) antibodyCYB5R3 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome B5 Reductase 3 (CYB5R3) antibodyCYB5R3 适用: 人, 犬, 猴 WB, IHC, FACS 宿主: 小鼠 Monoclonal 2A10 unconjugated
anti-Cytochrome B5 Reductase 3 (CYB5R3) (AA 125-334) antibodyCYB5R3 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome B5 Reductase 3 (CYB5R3) antibodyCYB5R3 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Cytochrome B5 Reductase 3 (CYB5R3) (AA 111-144) antibodyCYB5R3 适用: 人, 大鼠, 小鼠 WB 宿主: 兔 Polyclonal RB49795 unconjugated
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应用备注
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Peptide ELISA: Detection limit dilution 1/32000. Western blot: 0.1-1 μg/mL. Approx 35 kDa band observed in Mouse Liver lysates as well as inMouse and Rat brain lysates (calculated MW of 34.5 kDa according to Mouse NP_084063.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Species Reactivity: Tested: Mouse. Expected from sequence similarity: Rat, Canine (Dog) and Cow. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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: "Enhanced DNA adduct formation by benzo[a]pyrene in human liver cells lacking cytochrome P450 oxidoreductase." in: Mutation research, Vol. 852, pp. 503162, (2020) (PubMed).
: "Application of hepatic cytochrome b5/P450 reductase null (HBRN) mice to study the role of cytochrome b5 in the cytochrome P450-mediated bioactivation of the anticancer drug ellipticine." in: Toxicology and applied pharmacology, Vol. 366, pp. 64-74, (2019) (PubMed).
: "Cytochrome b 5 impacts on cytochrome P450-mediated metabolism of benzo[a]pyrene and its DNA adduct formation: studies in hepatic cytochrome b 5 /P450 reductase null (HBRN) mice." in: Archives of toxicology, Vol. 92, Issue 4, pp. 1625-1638, (2019) (PubMed).
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: "Enhanced DNA adduct formation by benzo[a]pyrene in human liver cells lacking cytochrome P450 oxidoreductase." in: Mutation research, Vol. 852, pp. 503162, (2020) (PubMed).
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- CYB5R3 (Cytochrome B5 Reductase 3 (CYB5R3))
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别名
- CYB5R3
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背景
- CYB5R3 belongs to the flavoprotein pyridine nucleotide cytochrome reductase family and contains 1 FAD-binding FR-type domain. There are two isoforms, NADH-cytochrome b5 reductase 3 membrane bound form and NADH-cytochrome b5 reductase 3 soluble form. Its functions include desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Defects in CYB5R3 are the cause of hereditary methemoglobinemia (HM). There are three forms of this disease: type 1 (HM1) in which the enzyme is only deficient in erythrocytes with a mild cyanosis, type 2 (HM2), in which the enzyme is completely deficient, type 3 (HM3) where the deficiency is seen in all blood cells. Type 2 is a severe form accompanied with mental retardation and neurological impairment.Synonyms: B5R, DIA1, Diaphorase-1, NADH-cytochrome b5 reductase 3
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基因ID
- 1727
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NCBI登录号
- NP_000389
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UniProt
- P00387
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途径
- SARS-CoV-2 Protein Interactome
抗原
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