Hsc70 抗体
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北京 101111
Quick Overview for Hsc70 抗体 (ABIN453949)
抗原
See all Hsc70 (HSPA8) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody detects 73 kDa protein corresponding to the molecular mass of hsc70 on SDS PAGE immunoblots. Does not cross react with hsp70.
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交叉反应 （详细）
- Species reactivity (tested):Human, Mouse, Rat
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产品特性
- Synonyms: HSP73, HSPA10, Heat shock cognate 71 kDa protein, Heat shock 70 kDa protein 8
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纯化方法
- Purified
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免疫原
- Human Hsc70
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亚型
- IgG2a
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anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 2-646) antibody
HSPA8 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 小鼠 Monoclonal 6E4D2 unconjugated
anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 2-646) antibodyHSPA8 适用: 人 WB, IHC, ELISA, IP, FACS 宿主: 小鼠 Monoclonal 2G8F6 unconjugated
anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 1-300) antibodyHSPA8 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 70kDa Protein 8 (HSPA8) (C-Term) antibodyHSPA8 适用: 人, 大鼠, 小鼠 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 70kDa Protein 8 (HSPA8) antibodyHSPA8 适用: 人 WB, IHC, ELISA, IP, ICC, IF, Bind, PLA, AA 宿主: 小鼠 Monoclonal 1F2-H5 unconjugated
anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 539-569), (C-Term) antibodyHSPA8 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB16528 unconjugated
anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 82-110), (N-Term) antibodyHSPA8 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB17655 unconjugated
anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 13-31), (N-Term) antibodyHSPA8 适用: 人, 大鼠, 小鼠 WB, IHC, ICC, IF, FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 520-614) antibodyHSPA8 适用: 人, 大鼠, 小鼠 WB, IHC, ICC, IF, FACS 宿主: 小鼠 Monoclonal 3B6 unconjugated
anti-Heat Shock 70kDa Protein 8 (HSPA8) (N-Term) antibodyHSPA8 适用: 人, 大鼠, 小鼠, Cow, 绵羊, Pig, 斑马鱼, 山羊 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Western blot: 1: 1000. ELISA. Immunoprecipitaion. Immunohistochemistry on frozen sections.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS pH 7.4, in 50 % glycerol and 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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- Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
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别名
- HSPA8 / HSC70
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背景
- Hsp70 genes encode abundant heat-inducible 70- kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). When cells are subjected to metabolic stress (e.g., heat shock) a member of the hsp 70 family, hsp 70 (hsp72), is expressed, hsp 70 is highly related to hsc70 (>90 % sequence identity). Constitutively expressed hsc70 rapidly forms a stable complex with the highly inducible hsp70 in cells following heat shock. The interaction of hsc70 with hsp 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on hsc70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (6).Synonyms: HSP73, HSPA10, Heat shock 70 kDa protein 8, Heat shock cognate 71 kDa protein
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基因ID
- 3312
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UniProt
- P11142
抗原
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