EIF5A 抗体 (Internal Region, N-Term)
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Quick Overview for EIF5A 抗体 (Internal Region, N-Term) (ABIN452675)
抗原
See all EIF5A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region, N-Term
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特异性
- Recognizes eIF5A.
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交叉反应 (详细)
- Species reactivity (tested):Human.
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纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-RPPHRASFLKRLESK, from the internal region (near N Terminus) of Human eIF5A.
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应用备注
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Peptide ELISA: eIF5A Antibody Detection limit dilution 1/128000. Western blot: 1-3 μg/mL. Approx 20 kDa band observed in MOLT4 lysates (calculated MW of20.0 kDa according to NP_001137232.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))
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别名
- EIF5A1
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背景
- Eukaryotic translation initiation factor 5A (eIF5A) is an 18 kDa protein, the only protein known to contain unusual amino acid formed by the action of deoxyhypusine synthase and deoxyhypusine hydroxylase using spermidine as the substrate. eIF5A protein was previously reported to be involved in the first step of peptide bond formation in translation, however more recent work implicates it as a universally conserved translation elongation factor. Modulation of eIF5A has been linked to proliferation and cancer.Synonyms: Eukaryotic initiation factor 5A isoform 1, Eukaryotic translation initiation factor 5A-1, Rev-binding factor, eIF-4D, eIF-5A, eIF-5A-1, eIF-5A1
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基因ID
- 1984
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NCBI登录号
- NP_001137232
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UniProt
- P63241
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途径
- Regulation of Muscle Cell Differentiation
抗原
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