Reactive to Serine383 phosphorylated Human ELK-1 peptide fragment conjugated with KLH or the peptide-BSA conjugate. Not reactive to unconjugated Serine383 phosphorylated Human ELK-1 peptide, KLH or BSA.
交叉反应 (详细)
Species reactivity (tested):Human.
产品特性
Synonyms: Elk-1, ETS domain-containing protein Elk-1
纯化方法
Affinity Chromatography on Protein G.
免疫原
Peptide of a phosphorylated fragment of ELK1 (pSer383) conjugated with KLH.
Indirect ELISA: The suggested antigen Coating quantity is 1 μg/mL of peptide/or peptideconjugating in pH 9.5 carbonate buffer, 100 μL/well. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL.
缓冲液
0.01 M PBS, pH 7.0 without preservatives
储存液
Without preservative
储存条件
-20 °C
储存方法
Store the antibody at -20 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
有效期
12 months
抗原
ELK1
(ELK1, Member of ETS Oncogene Family (ELK1))
别名
ELK1
背景
ELK1 belongs to the EST transcription factor family. Proteins in the family have a unique DNA binding domain that binds to conserved DNA sequences. Phosphorylation of ELK1 at Serine 383 and 389 by mitogen-activated protein kinases (MAPKs) activates ELK1. The binding of ELK1 to target sequences is also regulated by its co-factor, serum response factor (SRF), by alternative splicing, and by SUMOylation. ELK1 regulates the transcription of c-fos and genes encoding cytoskeleton proteins, and was found to play a role in longterm memory formation, drug addiction and other neurological disorders.Synonyms: ETS domain-containing protein Elk-1, Elk-1