Helicobacter Pylori 抗体
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北京 101111
Quick Overview for Helicobacter Pylori 抗体 (ABIN452543)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- Synonyms: H. pylori CagA Gene Product, Helicobacter pylori cytokine associated gene A
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纯化方法
- Affinity Chromatography on Protein G.
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免疫原
- A highly immunogenic 32Kd CagA fragment, recombinant.
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亚型
- IgG2a
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应用备注
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ELISA: HP CagA antibody (Clone 10E9) can be used as Capture antibody. In Sandwich ELISA, anti-HP CagA antibody clone 5H10 (Cat#ABIN452544) coated wellsmatches with HRP conjugated clone 5C6, clone 3C10 and clone 3C1 to detect therecombinant antigen. In addition, this clone selectively detected cell lysate of a CagA containing HP stain whenHRP conjugated clone 3C10 was used. Western Blotting: This clone detected a 32 KD band corresponding to the molecular weightof the recombinant CagA antigen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL.
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缓冲液
- 0.01 M PBS, pH 7.0 without preservatives
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储存液
- Without preservative
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储存条件
- -20 °C
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储存方法
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Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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- Helicobacter Pylori (H. pylori)
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别名
- Helicobacter Pylori
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物质类
- Virus
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背景
- Helicobacter pylori (H. pylori), a spiral rod shaped gram-negative bacterium, is frequently found in the stomach. In infected populations, 10-20 % may develop gastritis and gastric ulcer and 1-2 % may develop cancer. The genome of H. pylori isolates from carriers with symptoms contains a 40kb pathogenicity island encoding H. pylori cytotoxin, cytotoxin associated gene A protein (CagA) and other virulence associated factors. CagA is used as a biomarker for virulent H. pylori strains.Synonyms: H. pylori
抗原
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