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CD20 抗体 (C-Term)

Cited in 1 publication. The 山羊 多克隆 anti-CD20 antibody (ABIN401667) specifically detects CD20 in WB 和 EIA. The antibody is reactive with 人 samples.
产品编号 ABIN401667
发货至: 中国
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Quick Overview for CD20 抗体 (C-Term) (ABIN401667)

抗原

See all CD20 (MS4A1) 抗体
CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This CD20 antibody is un-conjugated

应用范围

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Western Blotting (WB), Enzyme Immunoassay (EIA)
  • 抗原表位

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    C-Term

    序列

    CQDQESSPIE NDSSP

    特异性

    This antibody recognizes CD20/MS4A1.

    交叉反应 (详细)

    Species reactivity (tested):Human.

    纯化方法

    Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence from the C Terminus of the protein sequence according to NP_068769.2, NP_690605.1. Genename: MS4A1
  • 应用备注

    Peptide ELISA: 1/8000. Western Blot: 3-5 μg/mL at Overnight incubation. Approx 37 kDa band seen in Daudi celllysates. Immunohistochemistry on Paraffin Sections: 2-5 μg/mL. In paraffin embedded Human Spleen, this antibody shows strong membranous staining inthe the follicles formed by B-cells.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    0.5 mg/mL

    缓冲液

    Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preserative and 0.5 % BSA as stabilizer.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Vargas-Inchaustegui, Demers, Shaw, Kang, Ball, Tuero, Musich, Mohanram, Demberg, Karpova, Li, Robert-Guroff: "Vaccine Induction of Lymph Node-Resident Simian Immunodeficiency Virus Env-Specific T Follicular Helper Cells in Rhesus Macaques." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 196, Issue 4, pp. 1700-10, (2016) (PubMed).

  • 抗原

    CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

    别名

    CD20

    背景

    CD20 is a non glycosylated protein with a molecular weight of 35 or 37 kDa depending on the degree of phosphorylation. Although not a member of the tetraspanin superfamily of cell surface receptors, it crosses the cell membrane four times. The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on normal T lymphocytes. The CD20 Molecule is involved in regulation of B cell differentiation, presumably via its reported function as a Ca++ channel subunit.Synonyms: B-cell marker, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leu-16, MS4A1, Membrane-spanning 4-domains subfamily A member 1

    基因ID

    931

    NCBI登录号

    NP_068769

    UniProt

    P11836
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