OCT4 抗体 (Internal Region)
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Quick Overview for OCT4 抗体 (Internal Region) (ABIN401447)
抗原
See all OCT4 (POU5F1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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特异性
- This antibody reacts with Oct-4 protein.
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交叉反应 （详细）
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Species reactivity (expected):Chimpanzee, Macaque, Horse, Mink, Canine, Bovine, Porcine.
Species reactivity (tested):Human, Mouse. -
纯化方法
- Affinity chromatography
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免疫原
- Synthetic peptide corresponding to an internal region of human Oct-4 protein
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亚型
- IgG
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anti-POU Class 5 Homeobox 1 (POU5F1) (AA 81-164) antibody
POU5F1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1B11 unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) antibodyKO Validated POU5F1 适用: 人 WB, ICC, IP, IF, FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 201-300) antibodyPOU5F1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 1-360) antibodyPOU5F1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 小鼠 Monoclonal 2H41D8 unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) antibodyPOU5F1 适用: 人 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 7E7 unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 136-360) antibodyPOU5F1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 1-360) antibodyPOU5F1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 81-164) antibodyPOU5F1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D2 unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) antibodyPOU5F1 适用: 人, 猴 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) antibodyPOU5F1 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB1425 unconjugated Recombinant Antibody
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应用备注
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ELISA: 1/350,000. Western blot: 1/1,000 - 1/1,500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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- OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
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别名
- OCT3/4
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背景
- Oct-4, also known as POU domain transcription factor, Octomer- binding transcription factor 3 and Oct-3, is expressed in human and mouse totipotent embryonic stem and germ cells. It may function in preventing these cells from differentiating. Each type of established mammalian pluripotent stem cell line (embryonal carcinoma (EC) cells, embryonic stem (ES) cells and embryonic germ (EG) cells) expresses Oct-4, which disappears rapidly when the cells differentiate. Most studies on Oct-4 have been performed on murine embryos. In human oocytes of various maturity and normally developing preimplantation/cleavage embryos, a variable Oct-4 expression pattern has been revealed, concomitant with a pure cytoplasmic localization of the protein. This variability in expression fades away during compaction, indicating embryonic Oct-4 expression, the protein appears in the nucleus, implying biological activity. Functional Oct-4 has been found in human amniotic fluid. As Oct-4 transcripts have also been found in various differentiated cells, the presence of Oct-4 transcripts or proteins may not be sufficient for identifying undifferentiated cell lines in humans. It may also be important to examine the localization of Oct-4 proteins within a cell. Oct-4 has been shown to interact with Nanog and with proteins from multiple repression complexes, including the NuRD, Sin3A and Pml complexes, possibly to control ES fate. Reactivation of Oct-4 expression is postulated to occur in differentiated cells that have undergone carcinogenesis, or tumor formation.Synonyms: OCT3, OCT4, OTF3, Oct-3, Oct-4, Octamer-binding transcription factor 3, POU domain, POU5F1, class 5, transcription factor 1
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基因ID
- 5460
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NCBI登录号
- NP_002692
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UniProt
- Q01860
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途径
- Stem Cell Maintenance
抗原
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