Aurora Kinase B 抗体 (pThr232)
Quick Overview for Aurora Kinase B 抗体 (pThr232) (ABIN401272)
抗原
See all Aurora Kinase B (AURKB) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 223-234, pThr232
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特异性
- The product was affinity purified from monospecific antiserum by Immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This antibody is directed against the phosphorylated form of human Aurora Kinase B at the pT232 residue. Reactivity with non-phosphorylated human Aurora Kinase B is minimal by ELISA. No reaction is expected against Aurora Kinase A. Cross reactivity with Aurora Kinase B from other sources is not known.
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交叉反应 （详细）
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Species reactivity (expected):Mouse, Rat, Bovine, Pig, Dog and Chimpanzee (100 %).
Species reactivity (tested):Human. -
纯化方法
- Affinity Chromatography.
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免疫原
- Synthetic peptide corresponding aa 223-234 of Human Aurora Kinase B protein
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亚型
- IgG
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anti-Aurora Kinase B (AURKB) (AA 1-90) antibody
AURKB 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 6H7 unconjugated
anti-Aurora Kinase B (AURKB) (Internal Region) antibodyAURKB 适用: 人, 猴 IHC, WB, ELISA, FM 宿主: 兔 Polyclonal unconjugated
anti-Aurora Kinase B (AURKB) (AA 6-35) antibodyAURKB 适用: 人, 小鼠, 大鼠, 灵长类 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Aurora Kinase B (AURKB) antibodyAURKB 适用: 人 IHC, WB, ELISA, IP 宿主: 兔 Monoclonal 4E5 unconjugated Recombinant Antibody
anti-Aurora Kinase B (AURKB) (AA 1-90) antibodyAURKB 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 6G8 unconjugated
anti-Aurora Kinase B (AURKB) (AA 89-251) antibodyAURKB 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal rAURKB-1592 unconjugated Recombinant Antibody
anti-Aurora Kinase B (AURKB) (pThr232) antibodyAURKB 适用: 人, 小鼠, 大鼠, 猴 IHC, WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Aurora Kinase B (AURKB) (AA 1-255) antibodyAURKB 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Aurora Kinase B (AURKB) (pThr232) antibodyAURKB 适用: 人, 小鼠, 大鼠, Cow, Pig IHC, WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Aurora Kinase B (AURKB) (AA 89-251) antibodyAURKB 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal AURKB-1592 unconjugated
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应用备注
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ELISA: 1/10,000-1/50,000. Western blot: 1/250-1/2,000 (See protocol below). Expect a band approx. 39 kDa in size corresponding to Aurora Kinase B by western blotting in theappropriate cell lysate or extract. HeLa cell lysate can be used as a positive control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.21 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Aurora Kinase B (AURKB)
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别名
- Aurora Kinase B
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背景
- Aurora Kinase B (also known as Aurora and Ipl1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, Aurora-related kinase 2, STK-1, and Aurora-B) is a Ser/Thr protein kinase member of the Aurora subfamily that may be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Aurora Kinase B is localized to the midzone of central spindle in late anaphase and concentrated into the midbody in telophase and cytokinesis and is colocalized with gamma tubulin in the mid-body. High levels of Aurora B expression are seen in the thymus, although it is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Aurora B is expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.Synonyms: AIK2, AIM1, ARK2, AURKB, Aurora- and Ipl1-like midbody-associated protein 1, Aurora-B, Aurora/IPL1-related kinase 2, STK-1, STK12, Serine/threonine-protein kinase 12
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基因ID
- 9212
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NCBI登录号
- NP_004208
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UniProt
- Q96GD4
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途径
- TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades
抗原
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