PNKP 抗体 (Internal Region)
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北京 101111
Quick Overview for PNKP 抗体 (Internal Region) (ABIN401253)
抗原
See all PNKP 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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特异性
- This affinity purified antibody is directed against PNK protein.
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交叉反应 （详细）
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Species reactivity (expected):Chimpanzee (100 %).Dog, mouse and rat (83 %).
Species reactivity (tested):Human. -
纯化方法
- Immunoaffinity chromatography
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免疫原
- Synthetic peptide corresponding to an internal region of human PNK protein
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亚型
- IgG
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anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 60-320) antibody
PNKP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) antibodyPNKP 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 422-521) antibodyPNKP 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 1-521) antibodyPNKP 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) antibodyPNKP 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB965 unconjugated Recombinant Antibody
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) antibodyPNKP 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB805 unconjugated Recombinant Antibody
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (N-Term) antibodyPNKP 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 1-243) antibodyPNKP 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 359-408) antibodyPNKP 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, Bat, Hamster, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (N-Term) antibodyPNKP 适用: 人, 小鼠, 犬, Cow, 豚鼠, 马, Bat, Hamster, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1: 3,000 - 1: 12,000. Western blot: 1: 300 - 1: 1,500 (Expect a band approximately 57 kDa in size corresponding toPNK in the appropriate cell lysate or extract).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.46 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody at 2 - 8 °C up to one month or for longer (in aliquots) at -20 °C or below.
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- PNKP (Polynucleotide Kinase 3'-Phosphatase (PNKP))
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别名
- PNKP
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背景
- Mammalian PNK (also known as PNKP, Polynucleotide kinase 3'-phosphatase, DNA 5'-kinase/3'- hosphatase and Polynucleotide kinase) catalyzes the 5' phosphorylation of nucleic acids and can have associated 3' phosphatase activity, predictive of an important function in DNA repair following ionizing radiation or oxidative damage. PNK is a nuclear protein that is expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine.Synonyms: Bifunctional polynucleotide phosphatase/kinase, DNA 5'-kinase/3'-phosphatase, Polynucleotide kinase-3'-phosphatase
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基因ID
- 11284
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NCBI登录号
- NP_009185
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UniProt
- Q96T60
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途径
- DNA Damage Repair, Nucleotide Phosphorylation
抗原
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