SFRP1 抗体
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北京 101111
Quick Overview for SFRP1 抗体 (ABIN401251)
抗原
See all SFRP1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This affinity purified antibody is directed against Human SFRP protein. A BLAST analysis was used to suggest reactivity with this protein from Human based on 100 % homology for the immunogen sequence. Expect cross reactivity with SFRP1 from Mouse, Rat and Bovine sources as only a single amino acid residue change is found within the immunogen sequence (91 % positive by BLAST). Cross reactivity with SFRP1 homologues from other sources has not been determined.
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交叉反应 （详细）
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Species reactivity (expected):Mouse, Rat and Bovine.
Species reactivity (tested):Human. -
纯化方法
- Immunoaffinity Chromatography
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免疫原
- Synthetic peptide corresponding to a 12 amino acid region of Human Sfrp1 protein
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亚型
- IgG
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anti-Secreted Frizzled-Related Protein 1 (SFRP1) (AA 8-153) antibody
SFRP1 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Secreted Frizzled-Related Protein 1 (SFRP1) (AA 201-314) antibodySFRP1 适用: 大鼠, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Secreted Frizzled-Related Protein 1 (SFRP1) (AA 32-314) antibodySFRP1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Secreted Frizzled-Related Protein 1 (SFRP1) (AA 32-314) antibodySFRP1 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C5 unconjugated
anti-Secreted Frizzled-Related Protein 1 (SFRP1) (AA 170-240) antibodySFRP1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Secreted Frizzled-Related Protein 1 (SFRP1) (AA 32-314) antibodySFRP1 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Secreted Frizzled-Related Protein 1 (SFRP1) (AA 26-55), (N-Term) antibodySFRP1 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB21557 unconjugated
anti-Secreted Frizzled-Related Protein 1 (SFRP1) antibodySFRP1 适用: 人 WB, IHC, ELISA, FM 宿主: 兔 Polyclonal unconjugated
anti-Secreted Frizzled-Related Protein 1 (SFRP1) (AA 33-63), (N-Term) antibodySFRP1 适用: 人 WB, IHC (p), FACS, EIA 宿主: 兔 Polyclonal unconjugated
anti-Secreted Frizzled-Related Protein 1 (SFRP1) antibodySFRP1 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
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应用备注
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ELISA: 1: 5,000 - 1: 25,000. Immunohistochemistry on paraffin sections: 1: 800. Western blot: 1: 200 - 1: 2,000 (Expect a band approximately 37 kDa in size corresponding toSfrp1 in the appropriate cell lysate or extract).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.56 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))
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别名
- SFRP1
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背景
- SFRP1 (also known as FRP, FRP1, SARP2, Secreted Apoptosis-related Protein 2, Secreted Frizzledrelated Protein and Secreted frizzled-related protein 1) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart.Synonyms: FRP, FRP-1, FRP1, SARP-2, SARP2, SFRP-1, Secreted apoptosis-related protein 2, Secreted frizzled-related protein 1
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分子量
- 35.4 kDa (Predicted)
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基因ID
- 6422
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NCBI登录号
- NP_003003
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UniProt
- Q8N474
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途径
- WNT signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of fat Cell Differentiation
抗原
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