KLH 抗体 (Alkaline Phosphatase (AP))
This anti-KLH antibody is a 兔 多克隆 antibody detecting KLH in WB, IHC (fro), EIA 和 DB. Suitable for Keyhole Limpet.
Quick Overview for KLH 抗体 (Alkaline Phosphatase (AP)) (ABIN401179)
抗原
See all KLH 抗体
KLH
(Keyhole Limpet Hemocyanin (KLH))
适用
Keyhole Limpet
宿主
兔
克隆类型
多克隆
标记
This KLH antibody is conjugated to Alkaline Phosphatase (AP)
应用范围
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Dot Blot (DB)
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纯化方法
- Affinity chromatography
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免疫原
- KLH (Keyhole Limpet Hemocyanin)
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亚型
- IgG
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anti-Keyhole Limpet Hemocyanin (KLH) antibody
KLH 适用: Keyhole Limpet ELISA, WB, IL 宿主: 兔 Polyclonal unconjugated
anti-Keyhole Limpet Hemocyanin (KLH) antibodyKLH 适用: Keyhole Limpet ELISA, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western BLot. ELISA: 1/3,000 - 1/13,500. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.005 M Magnesium Chloride, 0.0005 M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0 containing 10 mg/mL Bovine Serum Albumin (BSA) and 0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- DO NOT FREEZE!!
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C.
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- KLH (Keyhole Limpet Hemocyanin (KLH))
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背景
- KLH (Keyhole Limpet Hemocyanin) is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH. KLH forms a large complex composed of ~50 kDa subunits.Synonyms: Keyhole Limpet Hemocyanine
抗原
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