RNF34 抗体 (N-Term)
Quick Overview for RNF34 抗体 (N-Term) (ABIN401035)
抗原
See all RNF34 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-373, N-Term
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特异性
- This antibody is directed against RNF34 protein. Expect reactivity with isoform 1 and 2 of RNF34.
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交叉反应 （详细）
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Species reactivity (expected):Orangutan, Bovine, Canine, Mouse, Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity chromatography on Protein A
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免疫原
- Recombinant protein corresponding to amino acids 1-373 of human RNF34 protein
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亚型
- IgG
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anti-Ring Finger Protein 34 (RNF34) (AA 1-220) antibody
RNF34 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 34 (RNF34) (AA 1-373) antibodyRNF34 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Ring Finger Protein 34 (RNF34) (AA 143-192) antibodyRNF34 适用: 人, Cow, 兔, 豚鼠, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 34 (RNF34) (Middle Region) antibodyRNF34 适用: 人, Cow, 小鸡 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/4,000 - 1/20,000. Western Blot: 1/500 - 1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 0.1 mL of deionized water or equivalent.
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浓度
- 5.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- RNF34 (Ring Finger Protein 34 (RNF34))
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别名
- RFI / RNF34
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背景
- RNF34, also known as RING finger protein 34, RING finger protein RIFF, FYVE-RING finger protein Momo, Human RING finger homologous to inhibitor of apoptosis protein, hRFI, Caspases-8 and -10-associated RING finger protein 1, CARP-1 and Caspase regulator CARP1, is a novel modulator of NF-kappaB activation. RNF34 possesses E3 ubiquitin protein ligase activity and has been shown to regulate the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. RNF34 protects cells against apoptosis induced by TNF and may bind phosphatidylinositol phosphates. RNF34 is localized to both the nucleus and the cytoplasm. In the nucleus, a distinct speckled pattern is observed by immunofluorescence microscopy. In the cytoplasm, the protein is associated with membranes. RNF34 is expressed ubiquitously and can be detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes. Multiple transcript variants have been reported for this protein.Synonyms: CARP-1, CARP1, Caspases-8 and -10-associated RING finger protein 1, E3 ubiquitin-protein ligase RNF34, FYVE-RING finger protein Momo, Human RING finger homologous to inhibitor of apoptosis protein, RIFF
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基因ID
- 80196
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NCBI登录号
- NP_079402
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UniProt
- Q969K3
抗原
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