Mesothelin 抗体 (Extracellular Domain)
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北京 101111
Quick Overview for Mesothelin 抗体 (Extracellular Domain) (ABIN400817)
抗原
See all Mesothelin (MSLN) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Extracellular Domain
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特异性
- This antibody is directed against human mesothelin protein.
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交叉反应 （详细）
- Species reactivity (tested):Human.
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产品特性
- Synonyms: MSLN, MPF, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor
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纯化方法
- Protein A Chromatography.
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免疫原
- Recombinant protein corresponding to the extracellular domain of Human Mesothelin
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anti-Mesothelin (MSLN) (AA 273-407) antibody
MSLN 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 小鼠 Monoclonal MSLN-2131 unconjugated
anti-Mesothelin (MSLN) (AA 101-200) antibodyMSLN 适用: 人 WB, ELISA, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Mesothelin (MSLN) (AA 273-407) antibodyMSLN 适用: 人 IHC 宿主: 小鼠 Monoclonal MSLN-3384 unconjugated
anti-Mesothelin (MSLN) (AA 37-286) antibodyMSLN 适用: 人, 猴 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 5C11A4 unconjugated
anti-Mesothelin (MSLN) (AA 37-598) antibodyMSLN 适用: 人 ELISA, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Mesothelin (MSLN) (AA 273-407) antibody (Biotin)MSLN 适用: 人 IHC (f) 宿主: 小鼠 Monoclonal Biotin
anti-Mesothelin (MSLN) (AA 296-580) antibodyMSLN 适用: 人 ELISA, IHC, FACS 宿主: 兔 Monoclonal DM71 unconjugated
anti-Mesothelin (MSLN) (AA 37-286) antibodyMSLN 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 4H12F8 unconjugated
anti-Mesothelin (MSLN) (AA 296-606) antibodyMSLN 适用: 人, 大鼠 WB, ELISA 宿主: 小鼠 Monoclonal 7E6A6 unconjugated
anti-Mesothelin (MSLN) antibodyMSLN 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/10,000-1/50,000. Immunoprecipitaion. Western blot: 1/1,000. Expect a band approximately 40 kDa in size corresponding to mature mesothelin in theappropriate cell lysate or extract. Immunohistochemistry on Paraffin Sections: 1/100. Archival PEFF human tissues were deparaffinized followed by hydration. Antigen-retrievalis recommended. Block tissues with 1 % BSA in PBS for 30 min at 23 °C. Antibodies are diluted in 1 % BSA andreacted with tissue for 60 min at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C or below forlonger. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf life: one year from despatch. -
有效期
- 12 months
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- Mesothelin (MSLN)
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别名
- Mesothelin
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背景
- Mesothelin is a glycosyl-phosphatidylinositol-anchored glycoprotein present on the cell surface of various human solid tumors. The mesothelin (MSLN) gene encodes a 71- kDa precursor protein that is processed to a 40- kDa glycosylphosphatidylinositol-anchored protein that composes the mature portion and an NH2-terminal 31- kDa fragment called megakaryocyte-potentiating factor that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues, such as mesothelium, but aberrantly over expressed in mesotheliomas, ovarian, and pancreatic cancers. The biological functions of mesothelin remain elusive. A recent study showed that mesothelin binds to MUC16/CA125, and that this interaction mediates cell adhesion, suggesting that there may be an important role for MUC16/CA125 and mesothelin in the metastatic spread of ovarian cancer.Synonyms: CAK1 antigen, MPF, MSLN, Pre-pro-megakaryocyte-potentiating factor
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基因ID
- 10232
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UniProt
- Q13421
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途径
- EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process
抗原
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