MHV NSP9 抗体
The 小鼠 单克隆 anti-MHV NSP9 antibody is suitable to detect MHV NSP9 in samples from 小鼠. It has been validated for WB 和 IF.
产品编号 ABIN400814
发货至:
中国
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Our Local Distributor
中国
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北京 101111
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Quick Overview for MHV NSP9 抗体 (ABIN400814)
抗原
MHV NSP9
适用
小鼠
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Western Blotting (WB), Immunofluorescence (IF)
克隆位点
2C6-H1
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交叉反应 （详细）
- Species reactivity (tested):Mouse.
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产品特性
- Synonyms: Nonstructural Protein 9, Mouse Hepatitis Virus Strain A59, Murine coronavirus
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纯化方法
- Protein A chromatography
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免疫原
- E. coli derived fulllength MHV-A59 nsp9 protein. Remarks: This protein is part of the viral replicase polyprotein.
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亚型
- IgG2b
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anti-MHV NSP9 antibody
适用: SARS Coronavirus-2 (SARS-CoV-2) WB 宿主: 兔 Polyclonal unconjugated
anti-MHV NSP9 antibody适用: Mouse Hepatitis Virus (MHV) WB, FM 宿主: 小鼠 Monoclonal 2C6-H1 unconjugated
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应用备注
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Western blot: 1: 1,000 (Expect a band approximately 13 kDa in size corresponding to matureMHV-A59 nsp9 in the appropriate cell lysate or extract). Immunoflourescence: 1: 1,000 (Vero-E6 cells were grown on glass slides followed byinfection with MHVA59 strain and fixation with PBS/3 % PFA. After washing andpermeabilization of the fixed cells, antibody incubation was performed in PBS/5 %FCS for30 min). Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 2.1 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C or below for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate werecommend microcentrifugation before use.
Shelf life: one year from despatch. -
有效期
- 12 months
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- MHV NSP9
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物质类
- Viral Protein
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背景
- The nonstructural protein 9 (nsp9) is one of the Mouse hepatitis virus replicase cleavage products, encoded by ORF1a. Nsp9 is an RNA-binding protein that is thought to be part of the viral replication complex, which is associated with intracellular membranes.Synonyms: Mouse Hepatitis Virus Strain A59, Murine coronavirus, Nonstructural Protein 9
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UniProt
- P0C6X9
抗原
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