MHV NSP9 抗体
适用: Mouse Hepatitis Virus (MHV)
WB, FM
宿主: 小鼠
Monoclonal
2C6-H1
unconjugated
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- 抗原
- MHV NSP9
- 适用
- Mouse Hepatitis Virus (MHV)
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
- 应用范围
- Western Blotting (WB), Fluorescence Microscopy (FM)
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 免疫原
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This antibody was produced in mice by repeated immunizations with E.coli derived full-length MHV-A59 nsp9 protein. This protein is part of the viral replicase polyprotein.
Immunogentype:Native - 克隆位点
- 2C6-H1
- 亚型
- IgG
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anti-MHV NSP9 antibody
适用: SARS Coronavirus-2 (SARS-CoV-2) WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- This antibody has been tested for use in immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kDa in size corresponding to mature MHV-A59 nsp9 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells were grown on glass slides followed by infection with MHV-A59 strain and fixation with PBS/3%PFA. After washing and permeabilization of the fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 2.1 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
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- 抗原
- MHV NSP9
- 物质类
- Viral Protein
- 背景
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The nonstructural protein 9 (nsp9) is one of the Mouse hepatitis virus replicase cleavage products, encoded by ORF1a. Nsp9 is an RNA-binding protein that is thought to be part of the viral replication complex, which is associated with intracellular membranes.
Synonyms: MHV-A59 nsp9 protein antibody - 基因ID
- 1489749, 25121567
- UniProt
- P0C6V0
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