STAT5A 抗体 (Tyr694)
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北京 101111
Quick Overview for STAT5A 抗体 (Tyr694) (ABIN400812)
抗原
See all STAT5A 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Tyr694
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特异性
- This phospho-specific monoclonal antibody reacts with Stat5a pTyr694 and shows minimal reactivity by ELISA against the nonphosphorylated form of the immunizing peptide.
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交叉反应 （详细）
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Species reactivity (expected):Human and Rat (100 %).
Species reactivity (tested):Mouse. -
产品特性
- Synonyms: STAT-5, STAT-5A, Mammary gland factor, MGF, Signal transducer and activator oftranscription 5A
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纯化方法
- Protein A Chromatography from concentrated tissue culture supernate.
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免疫原
- Synthetic peptide corresponding to residues surrounding Tyr694 of Mouse STAT5a protein.
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亚型
- IgG1
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anti-Signal Transducer and Activator of Transcription 5A (STAT5A) antibody
STAT5A 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (cc) 宿主: 兔 Monoclonal 6E5 unconjugated
anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (AA 583-794) antibodySTAT5A 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 6D4 unconjugated
anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (AA 1-794) antibodySTAT5A 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (AA 744-794) antibodySTAT5A 适用: 人, 小鼠 WB, IHC, FACS, IP, ICC 宿主: 兔 Monoclonal BLR124H unconjugated Recombinant Antibody
anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (AA 1-104) antibodySTAT5A 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 1B12 unconjugated
anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (AA 635-794) antibodySTAT5A 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 5A (STAT5A) antibodyKD Validated STAT5A 适用: 人 WB, FACS, IF (p) 宿主: 兔 Monoclonal 24GB1920 unconjugated Recombinant Antibody
anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (pSer780) antibodySTAT5A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (pTyr694) antibodySTAT5A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (C-Term) antibodySTAT5A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/20,000. Western blot: 1/500-1/2,000 (Expect a band approximately 91 kDa in size corresponding tophosphorylated Stat5α protein in the appropriate cell lysate or extract). Immunohistochemistry (20-40 μg/mL). Immunofluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
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Store vial at -20 °C or below prior to opening. For low volume of reagent (25 μl), to minimizeloss of volume dilute 1/10 by adding 225 μl of the buffer stated abovedirectly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at thebottom of the vial. Use this intermediate dilution when calculating final dilutions asrecommended below. Store the vial at -20 °C or below after dilution. Avoid cycles of freezing and thawing.
Shelf life: One year from despatch (3 months from date of opening). -
有效期
- 12 months
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- STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))
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别名
- STAT5 / STAT5A
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背景
- Signal transducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic transcription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two isoforms, Stat5alpha and Stat5beta. Aberrant Stat5 activation has been implicated in the pathogenesis of chronic myelogenous leukemia, prostate and breast cancer and tumor metastasis. Stat5 is localized in the cytoplasm and upon phosphorylation at Y694 is translocated to the nucleus.Synonyms: MGF, Mammary gland factor, STAT-5, STAT-5A, Signal transducer and activator of transcription 5A
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基因ID
- 20850
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UniProt
- P42230
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途径
- JAK/STAT Signaling, RTK signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation
抗原
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