MYLK3 抗体 (N-Term)
(2 references)Quick Overview for MYLK3 抗体 (N-Term) (ABIN392494)
抗原
See all MYLK3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 40-69, N-Term
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纯化方法
- This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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免疫原
- This MYLK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-69 amino acids from the N-terminal region of human MYLK3.
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亚型
- Ig Fraction
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anti-Myosin Light Chain Kinase 3 (MYLK3) (AA 40-69) antibody
MYLK3 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase 3 (MYLK3) (AA 555-786) antibodyMYLK3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase 3 (MYLK3) (AA 1-240) antibodyMYLK3 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase 3 (MYLK3) antibodyMYLK3 适用: 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase 3 (MYLK3) (AA 488-750) antibodyMYLK3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase 3 (MYLK3) antibodyMYLK3 适用: 人, 小鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase 3 (MYLK3) (Internal Region) antibodyMYLK3 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase 3 (MYLK3) (AA 1-240) antibodyMYLK3 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100. IHC-P: 1:50~100
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
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有效期
- 6 months
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: "Interferon Stimulated Gene 15 upregulation precedes the development of blood brain barrier disruption and cerebral edema after traumatic brain injury in young mice." in: Journal of neurotrauma, (2015) (PubMed).
: "Albumin causes increased myosin light chain kinase expression in astrocytes via p38 mitogen-activated protein kinase." in: Journal of neuroscience research, Vol. 89, Issue 6, pp. 852-61, (2011) (PubMed).
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: "Interferon Stimulated Gene 15 upregulation precedes the development of blood brain barrier disruption and cerebral edema after traumatic brain injury in young mice." in: Journal of neurotrauma, (2015) (PubMed).
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- MYLK3 (Myosin Light Chain Kinase 3 (MYLK3))
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别名
- MYLK3
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背景
- MLCK, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.
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分子量
- 88393
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基因ID
- 91807
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NCBI登录号
- NP_872299
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UniProt
- Q32MK0
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途径
- Regulation of Muscle Cell Differentiation
抗原
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