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GAPDH 抗体 (N-Term)

Name: GAPDH 抗体 (N-Term)
SKU: ABIN392353
Availability: OutOfStock

GAPDH 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB16537 unconjugated

All lanes : Anti-GDH Antibody (N-term) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 μg per lane. (GAPDH 抗体 (N-Term))

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GDH antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (GAPDH 抗体 (N-Term))

IHC (p)

GDH Antibody (N-term) 7873a confocal immunofluorescent analysis with Hela cell. (GAPDH 抗体 (N-Term))

Confocal immunofluorescent analysis of GDH Antibody (N-term) 7873a with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (GAPDH 抗体 (N-Term))

Western blot analysis of lysate from cell line, using GDH Antibody (N-term) 7873a. (GAPDH 抗体 (N-Term))

Western blot analysis of lysates from Hela,HUVEC cell line (from left to right),using GDH Antibody (N-term) 7873a. (GAPDH 抗体 (N-Term))

6 images

(14 references)

产品编号 ABIN392353

发货至: 中国

Datasheet as PDF

抗原 See all GAPDH 抗体

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
抗原表位
34

22

13

12

7

6

6

5

4

4

3

3

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 62-91, N-Term
适用
212

147

130

64

46

39

25

25

23

19

19

17

15

15

14

13

10

9

8

6

4

4

4

4

4

4

4

3

3

2

2

2

2

1

1

1

1

1

1
人
宿主
159

88

16

9

4
兔
Compatible Secondaries
克隆类型
180

94
多克隆
标记
157

29

18

16

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1
This GAPDH antibody is un-conjugated
应用范围
221

103

71

65

37

33

30

27

16

15

15

15

7

6

5

4

3

3

1

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

纯化方法

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

免疫原

This GAPDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-91 amino acids from the N-terminal region of human GAPDH.

克隆位点

RB16537

亚型

Ig Fraction

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GAPDH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3C2 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibody
Verified GAPDH 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 136-335) antibody
GAPDH 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
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anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 2-148) antibody
GAPDH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 154-324) antibody
GAPDH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 3-335) antibody
GAPDH 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 2-148) antibody
GAPDH 适用: 人 WB 宿主: 小鼠 Monoclonal unconjugated

应用备注

IF: 1:10~50. IF: 1:10~50. WB: 1:1000. WB: 1:500. WB: 1:1000. IHC-P: 1:10~50

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Rao, Xia, Yang, Hu, Hu, Lei, Wu, Zhu: "Transient scrotal hyperthermia affects human sperm DNA integrity, sperm apoptosis, and sperm protein expression." in: Andrology, Vol. 4, Issue 6, pp. 1054-1063, (2017) (PubMed).

Shi, Qu, Li, Sui, Yao, Yang, Shi, Ji, Hou: "Increased expression of EHF via gene amplification contributes to the activation of HER family signaling and associates with poor survival in gastric cancer." in: Cell death & disease, Vol. 7, Issue 10, pp. e2442, (2017) (PubMed).

Yu, Lu, Han, Zhao, Li, Mao, Wang, Shen, Fan, Wang, Wang, Li, Tang, Song: "microRNA -140-5p inhibits colorectal cancer invasion and metastasis by targeting ADAMTS5 and IGFBP5." in: Stem cell research & therapy, Vol. 7, Issue 1, pp. 180, (2017) (PubMed).

Qiang, Sui, Ma, Li, Ren, Shao, Liu, Guan, Shi, Hou: "Proteasome inhibitor MG132 induces thyroid cancer cell apoptosis by modulating the activity of transcription factor FOXO3a." in: Endocrine, Vol. 56, Issue 1, pp. 98-108, (2017) (PubMed).

Zhou, Liu, Li, Zhang, Wang, Lan, Shi, Li, Ju: "Migration ability and Toll-like receptor expression of human mesenchymal stem cells improves significantly after three-dimensional culture." in: Biochemical and biophysical research communications, Vol. 491, Issue 2, pp. 323-328, (2017) (PubMed).

Ho, Morrison, Silva, Coomber: "The effect of 3-bromopyruvate on human colorectal cancer cells is dependent on glucose concentration but not hexokinase II expression." in: Bioscience reports, Vol. 36, Issue 1, pp. e00299, (2016) (PubMed).

Xu, Han, Tang, Zeng, Lin, Zhao, Zhang, Cao, Wu, Mao: "The Ring Finger Protein RNF6 Induces Leukemia Cell Proliferation as a Direct Target of Pre-B-cell Leukemia Homeobox 1." in: The Journal of biological chemistry, Vol. 291, Issue 18, pp. 9617-28, (2016) (PubMed).

Li, Lu, Wang, Han, Mao, Li, Yu, Wang, Fan, Yu, Song: "miR-221/222 enhance the tumorigenicity of human breast cancer stem cells via modulation of PTEN/Akt pathway." in: Biomedicine & pharmacotherapy, Vol. 79, pp. 93-101, (2016) (PubMed).

Wan, Qin, Zhang, Lu, Zhu, Zhang, Dai, Shi, Ye: "Oxidized low-density lipoprotein is associated with advanced-stage prostate cancer." in: Tumour biology, Vol. 36, Issue 5, pp. 3573-82, (2015) (PubMed).

Wang, Cui, Wang, Xue, Chen, Yu, Lu, Zhang, Tang, Peng: "Elevated kinesin family member 26B is a prognostic biomarker and a potential therapeutic target for colorectal cancer." in: Journal of experimental & clinical cancer research : CR, Vol. 34, pp. 13, (2015) (PubMed).

Li, Chen, Shi, Li, Cai, Zhou, Zhong, Yao: "Angiopoietin-like 4 enhances metastasis and inhibits apoptosis via inducing bone morphogenetic protein 7 in colorectal cancer cells." in: Biochemical and biophysical research communications, Vol. 467, Issue 1, pp. 128-34, (2015) (PubMed).

Zhu, Zhang, Li, Huang, Sun, Fu, Guan: "Prognostic significance of FAM3C in esophageal squamous cell carcinoma." in: Diagnostic pathology, Vol. 10, Issue 1, pp. 192, (2015) (PubMed).

He, Mangala, Theriot, Rohde, Wu, Zhang: "Cell killing and radiosensitizing effects of atorvastatin in PC3 prostate cancer cells." in: Journal of radiation research, Vol. 53, Issue 2, pp. 225-33, (2012) (PubMed).

Yang, Wang, Yu, Wang, Yuan, Zhang: "MicroRNAs are involved in erythroid differentiation control." in: Journal of cellular biochemistry, Vol. 107, Issue 3, pp. 548-56, (2009) (PubMed).
抗原

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

别名

GAPDH (GAPDH 产品)

背景

GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.

分子量

36053

基因ID

2597

NCBI登录号

NP_001243728, NP_002037

UniProt

P04406